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Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry

Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phas...

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Detalles Bibliográficos
Autores principales: Zhang, Yuwei, Lin, Xinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580109/
https://www.ncbi.nlm.nih.gov/pubmed/33111114
http://dx.doi.org/10.1016/j.xpro.2020.100080
Descripción
Sumario:Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification. For complete details on the use and execution of this protocol, please refer to Lin et al. (2020).