Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry

Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phas...

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Detalles Bibliográficos
Autores principales: Zhang, Yuwei, Lin, Xinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580109/
https://www.ncbi.nlm.nih.gov/pubmed/33111114
http://dx.doi.org/10.1016/j.xpro.2020.100080
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author Zhang, Yuwei
Lin, Xinda
author_facet Zhang, Yuwei
Lin, Xinda
author_sort Zhang, Yuwei
collection PubMed
description Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification. For complete details on the use and execution of this protocol, please refer to Lin et al. (2020).
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spelling pubmed-75801092020-10-26 Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry Zhang, Yuwei Lin, Xinda STAR Protoc Protocol Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification. For complete details on the use and execution of this protocol, please refer to Lin et al. (2020). Elsevier 2020-08-02 /pmc/articles/PMC7580109/ /pubmed/33111114 http://dx.doi.org/10.1016/j.xpro.2020.100080 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Yuwei
Lin, Xinda
Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title_full Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title_fullStr Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title_full_unstemmed Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title_short Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
title_sort protocol for analyzing cell cycle phases of polyphenic wings using flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580109/
https://www.ncbi.nlm.nih.gov/pubmed/33111114
http://dx.doi.org/10.1016/j.xpro.2020.100080
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