Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules

WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate potent, s...

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Detalles Bibliográficos
Autores principales: Chen, Hui, Lu, Chenggang, Lee, Sung-Jin, Li, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580111/
https://www.ncbi.nlm.nih.gov/pubmed/33111090
http://dx.doi.org/10.1016/j.xpro.2020.100043
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author Chen, Hui
Lu, Chenggang
Lee, Sung-Jin
Li, Yang
author_facet Chen, Hui
Lu, Chenggang
Lee, Sung-Jin
Li, Yang
author_sort Chen, Hui
collection PubMed
description WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate potent, soluble, selective WNT mimetics with drug-like properties for both research and therapeutic applications. To help researchers adapt and expand on this platform, we describe these protocols and key considerations in generating and studying WNT mimetics. For complete details on the use and execution of this protocol, please refer to Chen et al., 2020.
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spelling pubmed-75801112020-10-26 Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules Chen, Hui Lu, Chenggang Lee, Sung-Jin Li, Yang STAR Protoc Protocol WNT signaling is crucial for embryonic development, adult tissue homeostasis, and injury repair. The poor biophysical characteristics of WNTs and their lack of receptor selectivity have hindered their use as research tools or potential therapeutics. We have developed a platform to generate potent, soluble, selective WNT mimetics with drug-like properties for both research and therapeutic applications. To help researchers adapt and expand on this platform, we describe these protocols and key considerations in generating and studying WNT mimetics. For complete details on the use and execution of this protocol, please refer to Chen et al., 2020. Elsevier 2020-06-03 /pmc/articles/PMC7580111/ /pubmed/33111090 http://dx.doi.org/10.1016/j.xpro.2020.100043 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Hui
Lu, Chenggang
Lee, Sung-Jin
Li, Yang
Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_full Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_fullStr Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_full_unstemmed Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_short Protocol to Generate and Characterize Potent and Selective WNT Mimetic Molecules
title_sort protocol to generate and characterize potent and selective wnt mimetic molecules
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580111/
https://www.ncbi.nlm.nih.gov/pubmed/33111090
http://dx.doi.org/10.1016/j.xpro.2020.100043
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