Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies

Vagal sensory neurons relay viscero- and somatosensory information from within the body and play a key role in maintaining physiological homeostasis. We recently characterized the diversity of vagal sensory neurons in the mouse using a single-cell transcriptomics approach. Here, we provide an in-dep...

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Detalles Bibliográficos
Autores principales: Häring, Martin, Fatt, Michael, Kupari, Jussi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580114/
https://www.ncbi.nlm.nih.gov/pubmed/33111081
http://dx.doi.org/10.1016/j.xpro.2020.100030
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author Häring, Martin
Fatt, Michael
Kupari, Jussi
author_facet Häring, Martin
Fatt, Michael
Kupari, Jussi
author_sort Häring, Martin
collection PubMed
description Vagal sensory neurons relay viscero- and somatosensory information from within the body and play a key role in maintaining physiological homeostasis. We recently characterized the diversity of vagal sensory neurons in the mouse using a single-cell transcriptomics approach. Here, we provide an in-depth protocol for the extraction of mouse vagal ganglia and the production of high-quality single-cell suspensions from this tissue. This effective protocol can also be applied for use with other peripheral and central neuron populations with few modifications. For complete details on the use and execution of this protocol, please refer to Kupari et al. (2019).
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spelling pubmed-75801142020-10-26 Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies Häring, Martin Fatt, Michael Kupari, Jussi STAR Protoc Protocol Vagal sensory neurons relay viscero- and somatosensory information from within the body and play a key role in maintaining physiological homeostasis. We recently characterized the diversity of vagal sensory neurons in the mouse using a single-cell transcriptomics approach. Here, we provide an in-depth protocol for the extraction of mouse vagal ganglia and the production of high-quality single-cell suspensions from this tissue. This effective protocol can also be applied for use with other peripheral and central neuron populations with few modifications. For complete details on the use and execution of this protocol, please refer to Kupari et al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7580114/ /pubmed/33111081 http://dx.doi.org/10.1016/j.xpro.2020.100030 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Häring, Martin
Fatt, Michael
Kupari, Jussi
Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title_full Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title_fullStr Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title_full_unstemmed Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title_short Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies
title_sort protocol to prepare single-cell suspensions from mouse vagal sensory ganglia for transcriptomic studies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580114/
https://www.ncbi.nlm.nih.gov/pubmed/33111081
http://dx.doi.org/10.1016/j.xpro.2020.100030
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