Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons

This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. Th...

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Detalles Bibliográficos
Autores principales: Peitz, Michael, Krutenko, Tamara, Brüstle, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580116/
https://www.ncbi.nlm.nih.gov/pubmed/33111086
http://dx.doi.org/10.1016/j.xpro.2020.100038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580116/
https://www.ncbi.nlm.nih.gov/pubmed/33111086
http://dx.doi.org/10.1016/j.xpro.2020.100038

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