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Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons
This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. Th...
Autores principales: | Peitz, Michael, Krutenko, Tamara, Brüstle, Oliver |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580116/ https://www.ncbi.nlm.nih.gov/pubmed/33111086 http://dx.doi.org/10.1016/j.xpro.2020.100038 |
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