Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes

The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Kleinsorge, Mandy, Cyganek, Lukas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580117/
https://www.ncbi.nlm.nih.gov/pubmed/33111079
http://dx.doi.org/10.1016/j.xpro.2020.100026
Descripción
Sumario:The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.

Ejemplares similares