Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes

The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient mo...

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Detalles Bibliográficos
Autores principales: Kleinsorge, Mandy, Cyganek, Lukas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580117/
https://www.ncbi.nlm.nih.gov/pubmed/33111079
http://dx.doi.org/10.1016/j.xpro.2020.100026
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author Kleinsorge, Mandy
Cyganek, Lukas
author_facet Kleinsorge, Mandy
Cyganek, Lukas
author_sort Kleinsorge, Mandy
collection PubMed
description The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.
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spelling pubmed-75801172020-10-26 Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes Kleinsorge, Mandy Cyganek, Lukas STAR Protoc Protocol The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018. Elsevier 2020-06-03 /pmc/articles/PMC7580117/ /pubmed/33111079 http://dx.doi.org/10.1016/j.xpro.2020.100026 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kleinsorge, Mandy
Cyganek, Lukas
Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title_full Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title_fullStr Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title_full_unstemmed Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title_short Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes
title_sort subtype-directed differentiation of human ipscs into atrial and ventricular cardiomyocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580117/
https://www.ncbi.nlm.nih.gov/pubmed/33111079
http://dx.doi.org/10.1016/j.xpro.2020.100026
work_keys_str_mv AT kleinsorgemandy subtypedirecteddifferentiationofhumanipscsintoatrialandventricularcardiomyocytes
AT cyganeklukas subtypedirecteddifferentiationofhumanipscsintoatrialandventricularcardiomyocytes

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