Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient

Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tis...

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Autores principales: Tarazón, Estefanía, Portolés, Manuel, Roselló-Lletí, Esther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580119/
https://www.ncbi.nlm.nih.gov/pubmed/33111127
http://dx.doi.org/10.1016/j.xpro.2020.100100
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author Tarazón, Estefanía
Portolés, Manuel
Roselló-Lletí, Esther
author_facet Tarazón, Estefanía
Portolés, Manuel
Roselló-Lletí, Esther
author_sort Tarazón, Estefanía
collection PubMed
description Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tissue using a discontinuous sucrose gradient. For complete details on the use and execution of this protocol, please refer to Tarazon et al. (2017).
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spelling pubmed-75801192020-10-26 Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient Tarazón, Estefanía Portolés, Manuel Roselló-Lletí, Esther STAR Protoc Protocol Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tissue using a discontinuous sucrose gradient. For complete details on the use and execution of this protocol, please refer to Tarazon et al. (2017). Elsevier 2020-09-10 /pmc/articles/PMC7580119/ /pubmed/33111127 http://dx.doi.org/10.1016/j.xpro.2020.100100 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tarazón, Estefanía
Portolés, Manuel
Roselló-Lletí, Esther
Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title_full Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title_fullStr Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title_full_unstemmed Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title_short Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient
title_sort protocol for isolation of golgi vesicles from human and animal hearts by flotation through a discontinuous sucrose gradient
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580119/
https://www.ncbi.nlm.nih.gov/pubmed/33111127
http://dx.doi.org/10.1016/j.xpro.2020.100100
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