Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids

This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In human colon organoids, the function of cystic fibrosis transmembrane conductance regulator (CFTR) protein and its rescue by CFTR modulators can...

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Detalles Bibliográficos
Autores principales: Vonk, Annelotte M., van Mourik, Peter, Ramalho, Anabela S., Silva, Iris A.L., Statia, Marvin, Kruisselbrink, Evelien, Suen, Sylvia W.F., Dekkers, Johanna F., Vleggaar, Frank P., Houwen, Roderick H.J., Mullenders, Jasper, Boj, Sylvia F., Vries, Robert, Amaral, Margarida D., de Boeck, Kris, van der Ent, Cornelis K., Beekman, Jeffrey M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580120/
https://www.ncbi.nlm.nih.gov/pubmed/33111074
http://dx.doi.org/10.1016/j.xpro.2020.100019

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