Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is no...

Descripción completa

Detalles Bibliográficos
Autores principales: Saito, Yoshimasa, Muramatsu, Toshihide, Saito, Hidetsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580121/
https://www.ncbi.nlm.nih.gov/pubmed/33111070
http://dx.doi.org/10.1016/j.xpro.2019.100009
_version_ 1783598727821262848
author Saito, Yoshimasa
Muramatsu, Toshihide
Saito, Hidetsugu
author_facet Saito, Yoshimasa
Muramatsu, Toshihide
Saito, Hidetsugu
author_sort Saito, Yoshimasa
collection PubMed
description This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.
format Online
Article
Text
id pubmed-7580121
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75801212020-10-26 Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma Saito, Yoshimasa Muramatsu, Toshihide Saito, Hidetsugu STAR Protoc Protocol This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports. Elsevier 2020-06-03 /pmc/articles/PMC7580121/ /pubmed/33111070 http://dx.doi.org/10.1016/j.xpro.2019.100009 Text en © 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Saito, Yoshimasa
Muramatsu, Toshihide
Saito, Hidetsugu
Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title_full Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title_fullStr Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title_full_unstemmed Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title_short Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma
title_sort establishment and long-term culture of organoids derived from human biliary tract carcinoma
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580121/
https://www.ncbi.nlm.nih.gov/pubmed/33111070
http://dx.doi.org/10.1016/j.xpro.2019.100009
work_keys_str_mv AT saitoyoshimasa establishmentandlongtermcultureoforganoidsderivedfromhumanbiliarytractcarcinoma
AT muramatsutoshihide establishmentandlongtermcultureoforganoidsderivedfromhumanbiliarytractcarcinoma
AT saitohidetsugu establishmentandlongtermcultureoforganoidsderivedfromhumanbiliarytractcarcinoma

Ejemplares similares