CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming and inefficient. Here, we provide a detailed protocol for genetic manipulation, including gene deletion, insertion, and point mutation in A. baumannii using the platform. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).