Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells

Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary...

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Detalles Bibliográficos
Autores principales: Hor, Pooja, Ichida, Justin K., Borok, Zea, Ryan, Amy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580194/
https://www.ncbi.nlm.nih.gov/pubmed/33111106
http://dx.doi.org/10.1016/j.xpro.2020.100068
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author Hor, Pooja
Ichida, Justin K.
Borok, Zea
Ryan, Amy L.
author_facet Hor, Pooja
Ichida, Justin K.
Borok, Zea
Ryan, Amy L.
author_sort Hor, Pooja
collection PubMed
description Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction. For complete details on the use and execution of this protocol, please refer to Hor et al. (2020).
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spelling pubmed-75801942020-10-26 Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells Hor, Pooja Ichida, Justin K. Borok, Zea Ryan, Amy L. STAR Protoc Protocol Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction. For complete details on the use and execution of this protocol, please refer to Hor et al. (2020). Elsevier 2020-07-24 /pmc/articles/PMC7580194/ /pubmed/33111106 http://dx.doi.org/10.1016/j.xpro.2020.100068 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hor, Pooja
Ichida, Justin K.
Borok, Zea
Ryan, Amy L.
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_full Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_fullStr Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_full_unstemmed Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_short Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
title_sort protocol for differentiation of human ipscs into pulmonary neuroendocrine cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580194/
https://www.ncbi.nlm.nih.gov/pubmed/33111106
http://dx.doi.org/10.1016/j.xpro.2020.100068
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