Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation effic...

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Detalles Bibliográficos
Autores principales: Liu, Li-Ping, Guo, Ning-Ning, Li, Yu-Mei, Zheng, Yun-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580196/
https://www.ncbi.nlm.nih.gov/pubmed/33111066
http://dx.doi.org/10.1016/j.xpro.2019.100004
Descripción
Sumario:Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

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