Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation effic...

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Autores principales: Liu, Li-Ping, Guo, Ning-Ning, Li, Yu-Mei, Zheng, Yun-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580196/
https://www.ncbi.nlm.nih.gov/pubmed/33111066
http://dx.doi.org/10.1016/j.xpro.2019.100004
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author Liu, Li-Ping
Guo, Ning-Ning
Li, Yu-Mei
Zheng, Yun-Wen
author_facet Liu, Li-Ping
Guo, Ning-Ning
Li, Yu-Mei
Zheng, Yun-Wen
author_sort Liu, Li-Ping
collection PubMed
description Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).
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spelling pubmed-75801962020-10-26 Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System Liu, Li-Ping Guo, Ning-Ning Li, Yu-Mei Zheng, Yun-Wen STAR Protoc Protocol Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7580196/ /pubmed/33111066 http://dx.doi.org/10.1016/j.xpro.2019.100004 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Li-Ping
Guo, Ning-Ning
Li, Yu-Mei
Zheng, Yun-Wen
Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title_full Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title_fullStr Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title_full_unstemmed Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title_short Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
title_sort generation of human imelanocytes from induced pluripotent stem cells through a suspension culture system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580196/
https://www.ncbi.nlm.nih.gov/pubmed/33111066
http://dx.doi.org/10.1016/j.xpro.2019.100004
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