Cargando…

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation effic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Li-Ping, Guo, Ning-Ning, Li, Yu-Mei, Zheng, Yun-Wen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580196/ https://www.ncbi.nlm.nih.gov/pubmed/33111066 http://dx.doi.org/10.1016/j.xpro.2019.100004

Ejemplares similares

Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
por: Pollock, Samuel D., et al.
Publicado: (2023)

Generation of melanocytes from Induced pluripotent stem cells
por: Yang, Ruifeng, et al.
Publicado: (2011)

Generation of Human Melanocytes from Induced Pluripotent Stem Cells
por: Ohta, Shigeki, et al.
Publicado: (2011)

Generation of glutamatergic/GABAergic neuronal co-cultures derived from human induced pluripotent stem cells for characterizing E/I balance in vitro
por: Wang, Shan, et al.
Publicado: (2023)

Suspension culture improves iPSC expansion and pluripotency phenotype
por: Cuesta-Gomez, Nerea, et al.
Publicado: (2023)

Organotypic Human Skin Cultures Incorporating Primary Melanocytes
por: Roth‐Carter, Quinn R., et al.
Publicado: (2022)

Protocol to generate cardiac pericytes from human induced pluripotent stem cells
por: Shen, Mengcheng, et al.
Publicado: (2023)

The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture
por: Tang, Xianglian, et al.
Publicado: (2021)

Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
por: Li, Ronghui, et al.
Publicado: (2021)

Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
por: Santos, Renata, et al.
Publicado: (2022)

Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures
por: Fluri, David A., et al.
Publicado: (2012)

Early retinal differentiation of human pluripotent stem cells in microwell suspension cultures
por: Sharma, Vishal S., et al.
Publicado: (2016)

Kinetic modeling of human induced pluripotent stem cell expansion in suspension culture
por: Galvanauskas, Vytautas, et al.
Publicado: (2019)

Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
por: Castro-Viñuelas, Rocío, et al.
Publicado: (2021)

Assessing Cell Competition in Human Pluripotent Stem Cell (hPSC) Cultures
por: Price, Christopher J., et al.
Publicado: (2022)

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
por: Kim, Hyosung, et al.
Publicado: (2021)

Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
por: Nafria, Monica, et al.
Publicado: (2020)

Human‐Induced Pluripotent Stem Cell Culture Methods Under cGMP Conditions
por: Rivera, Teresa, et al.
Publicado: (2020)

Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture
por: Kempf, Henning, et al.
Publicado: (2014)

Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture
por: Olmer, Ruth, et al.
Publicado: (2018)

Generation of highly pure motor neurons from human induced pluripotent stem cells
por: Akter, Masuma, et al.
Publicado: (2022)

Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregates
por: Wijesekara, Piyumi, et al.
Publicado: (2023)

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells
por: Rohani, Leili, et al.
Publicado: (2020)

Generation and characterization of bat-induced pluripotent stem cells
por: Mo, Xiaohui, et al.
Publicado: (2014)

An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells
por: Lyra-Leite, Davi Marco, et al.
Publicado: (2021)

Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells
por: Chen, Luoman, et al.
Publicado: (2020)

Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells
por: Cao, Xu, et al.
Publicado: (2020)

An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
por: Haque, Mohammad, et al.
Publicado: (2021)

Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization
por: Anastasaki, Corina, et al.
Publicado: (2022)

Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
por: Sheta, Razan, et al.
Publicado: (2023)

Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoids
por: Feng, Yilin, et al.
Publicado: (2023)

Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture
por: Shinohara, Marie, et al.
Publicado: (2019)

Size-dependent hepatic differentiation of human induced pluripotent stem cells spheroid in suspension culture
por: Torizal, Fuad Gandhi, et al.
Publicado: (2019)

A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells
por: Sivalingam, Jaichandran, et al.
Publicado: (2020)

Generation of functional cardiomyocytes from rat embryonic and induced pluripotent stem cells using feeder-free expansion and differentiation in suspension culture
por: Dahlmann, Julia, et al.
Publicado: (2018)

Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays
por: Tan, Lay Shuen, et al.
Publicado: (2021)

High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
por: Sarrafha, Lily, et al.
Publicado: (2021)

Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells
por: Xi, Haibin, et al.
Publicado: (2020)

Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions
por: Perriot, Sylvain, et al.
Publicado: (2022)

Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
por: Sharma, Ruchi, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_bvafbbtggn9ujbc1aq5i8tf5c2