Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy

Single-cell analysis of tumor-infiltrating lymphocytes obtained before and after preoperative therapy reflects the dynamic interplay of the tumor and immune system during treatment. Here, we present a protocol to implement single-cell analysis of tumor-infiltrating B cells, which were isolated from...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Yiwen, Liao, Jian-You, Su, Shicheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580197/
https://www.ncbi.nlm.nih.gov/pubmed/33111088
http://dx.doi.org/10.1016/j.xpro.2020.100040
_version_ 1783598743117889536
author Lu, Yiwen
Liao, Jian-You
Su, Shicheng
author_facet Lu, Yiwen
Liao, Jian-You
Su, Shicheng
author_sort Lu, Yiwen
collection PubMed
description Single-cell analysis of tumor-infiltrating lymphocytes obtained before and after preoperative therapy reflects the dynamic interplay of the tumor and immune system during treatment. Here, we present a protocol to implement single-cell analysis of tumor-infiltrating B cells, which were isolated from paired human breast cancers before and after neo-adjuvant chemotherapy. This protocol also facilitates isolation and single-cell analysis of other tumor-infiltrating lymphocytes. For complete information on the generation and use of this protocol, please refer to Lu et al. (2020).
format Online
Article
Text
id pubmed-7580197
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75801972020-10-26 Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy Lu, Yiwen Liao, Jian-You Su, Shicheng STAR Protoc Protocol Single-cell analysis of tumor-infiltrating lymphocytes obtained before and after preoperative therapy reflects the dynamic interplay of the tumor and immune system during treatment. Here, we present a protocol to implement single-cell analysis of tumor-infiltrating B cells, which were isolated from paired human breast cancers before and after neo-adjuvant chemotherapy. This protocol also facilitates isolation and single-cell analysis of other tumor-infiltrating lymphocytes. For complete information on the generation and use of this protocol, please refer to Lu et al. (2020). Elsevier 2020-06-03 /pmc/articles/PMC7580197/ /pubmed/33111088 http://dx.doi.org/10.1016/j.xpro.2020.100040 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lu, Yiwen
Liao, Jian-You
Su, Shicheng
Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title_full Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title_fullStr Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title_full_unstemmed Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title_short Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy
title_sort protocol for single-cell analysis of tumor-infiltrating b cells isolated from human breast cancer tissue before and after neo-adjuvant chemotherapy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580197/
https://www.ncbi.nlm.nih.gov/pubmed/33111088
http://dx.doi.org/10.1016/j.xpro.2020.100040
work_keys_str_mv AT luyiwen protocolforsinglecellanalysisoftumorinfiltratingbcellsisolatedfromhumanbreastcancertissuebeforeandafterneoadjuvantchemotherapy
AT liaojianyou protocolforsinglecellanalysisoftumorinfiltratingbcellsisolatedfromhumanbreastcancertissuebeforeandafterneoadjuvantchemotherapy
AT sushicheng protocolforsinglecellanalysisoftumorinfiltratingbcellsisolatedfromhumanbreastcancertissuebeforeandafterneoadjuvantchemotherapy

Ejemplares similares