Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome

Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource f...

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Detalles Bibliográficos
Autores principales: Büll, Christian, Joshi, Hiren J., Clausen, Henrik, Narimatsu, Yoshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580198/
https://www.ncbi.nlm.nih.gov/pubmed/33111073
http://dx.doi.org/10.1016/j.xpro.2020.100017
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author Büll, Christian
Joshi, Hiren J.
Clausen, Henrik
Narimatsu, Yoshiki
author_facet Büll, Christian
Joshi, Hiren J.
Clausen, Henrik
Narimatsu, Yoshiki
author_sort Büll, Christian
collection PubMed
description Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays. For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
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spelling pubmed-75801982020-10-26 Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome Büll, Christian Joshi, Hiren J. Clausen, Henrik Narimatsu, Yoshiki STAR Protoc Protocol Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays. For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019). Elsevier 2020-06-03 /pmc/articles/PMC7580198/ /pubmed/33111073 http://dx.doi.org/10.1016/j.xpro.2020.100017 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Büll, Christian
Joshi, Hiren J.
Clausen, Henrik
Narimatsu, Yoshiki
Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title_full Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title_fullStr Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title_full_unstemmed Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title_short Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
title_sort cell-based glycan arrays—a practical guide to dissect the human glycome
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580198/
https://www.ncbi.nlm.nih.gov/pubmed/33111073
http://dx.doi.org/10.1016/j.xpro.2020.100017
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