Generation of Engineered Human Myocardium in a Multi-well Format

This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart muscle can be obtained to serve numerous...

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Detalles Bibliográficos
Autores principales: Tiburcy, Malte, Meyer, Tim, Liaw, Norman Y., Zimmermann, Wolfram-Hubertus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580201/
https://www.ncbi.nlm.nih.gov/pubmed/33111083
http://dx.doi.org/10.1016/j.xpro.2020.100032
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author Tiburcy, Malte
Meyer, Tim
Liaw, Norman Y.
Zimmermann, Wolfram-Hubertus
author_facet Tiburcy, Malte
Meyer, Tim
Liaw, Norman Y.
Zimmermann, Wolfram-Hubertus
author_sort Tiburcy, Malte
collection PubMed
description This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart muscle can be obtained to serve numerous applications, including drug screening and disease modelling. This protocol has been successfully applied to human embryonic stem (HES) cell- and induced PSC-derived cardiomyocytes, subtype-specific, i.e., atrial and ventricular, and commercially available cardiomyocyte preparations. For complete details on the use and execution of this protocol, please refer to Tiburcy et al. (2017).
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spelling pubmed-75802012020-10-26 Generation of Engineered Human Myocardium in a Multi-well Format Tiburcy, Malte Meyer, Tim Liaw, Norman Y. Zimmermann, Wolfram-Hubertus STAR Protoc Protocol This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart muscle can be obtained to serve numerous applications, including drug screening and disease modelling. This protocol has been successfully applied to human embryonic stem (HES) cell- and induced PSC-derived cardiomyocytes, subtype-specific, i.e., atrial and ventricular, and commercially available cardiomyocyte preparations. For complete details on the use and execution of this protocol, please refer to Tiburcy et al. (2017). Elsevier 2020-06-03 /pmc/articles/PMC7580201/ /pubmed/33111083 http://dx.doi.org/10.1016/j.xpro.2020.100032 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Tiburcy, Malte
Meyer, Tim
Liaw, Norman Y.
Zimmermann, Wolfram-Hubertus
Generation of Engineered Human Myocardium in a Multi-well Format
title Generation of Engineered Human Myocardium in a Multi-well Format
title_full Generation of Engineered Human Myocardium in a Multi-well Format
title_fullStr Generation of Engineered Human Myocardium in a Multi-well Format
title_full_unstemmed Generation of Engineered Human Myocardium in a Multi-well Format
title_short Generation of Engineered Human Myocardium in a Multi-well Format
title_sort generation of engineered human myocardium in a multi-well format
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580201/
https://www.ncbi.nlm.nih.gov/pubmed/33111083
http://dx.doi.org/10.1016/j.xpro.2020.100032
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