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Protocol for Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 System
This protocol uses endonuclease-dead, programmable RNA-guided RNA-targeting Cas13 RNases (d)Cas13 proteins fused with fluorescent proteins to visualize and track RNA dynamics in live cells. This protocol details several aspects of the procedure, including gRNA design, fluorescent protein selection,...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580206/ https://www.ncbi.nlm.nih.gov/pubmed/33111085 http://dx.doi.org/10.1016/j.xpro.2020.100037 |