Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation

Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-...

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Detalles Bibliográficos
Autores principales: Bakhurin, Konstantin I., Hughes, Ryan N., Barter, Joseph W., Zhang, Jinyong, Yin, Henry H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580214/
https://www.ncbi.nlm.nih.gov/pubmed/33111123
http://dx.doi.org/10.1016/j.xpro.2020.100091
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author Bakhurin, Konstantin I.
Hughes, Ryan N.
Barter, Joseph W.
Zhang, Jinyong
Yin, Henry H.
author_facet Bakhurin, Konstantin I.
Hughes, Ryan N.
Barter, Joseph W.
Zhang, Jinyong
Yin, Henry H.
author_sort Bakhurin, Konstantin I.
collection PubMed
description Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-fixation device that measures the forces exerted by head-fixed mice in multiple dimensions. We further detail how this system can be used in conjunction with in vivo electrophysiology and optogenetics to study dopamine neurons in the ventral tegmental area. For complete details on the use and execution of this protocol, please refer to Hughes et al. (2020a, 2020b)
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spelling pubmed-75802142020-10-26 Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation Bakhurin, Konstantin I. Hughes, Ryan N. Barter, Joseph W. Zhang, Jinyong Yin, Henry H. STAR Protoc Protocol Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-fixation device that measures the forces exerted by head-fixed mice in multiple dimensions. We further detail how this system can be used in conjunction with in vivo electrophysiology and optogenetics to study dopamine neurons in the ventral tegmental area. For complete details on the use and execution of this protocol, please refer to Hughes et al. (2020a, 2020b) Elsevier 2020-08-25 /pmc/articles/PMC7580214/ /pubmed/33111123 http://dx.doi.org/10.1016/j.xpro.2020.100091 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bakhurin, Konstantin I.
Hughes, Ryan N.
Barter, Joseph W.
Zhang, Jinyong
Yin, Henry H.
Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title_full Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title_fullStr Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title_full_unstemmed Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title_short Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation
title_sort protocol for recording from ventral tegmental area dopamine neurons in mice while measuring force during head-fixation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580214/
https://www.ncbi.nlm.nih.gov/pubmed/33111123
http://dx.doi.org/10.1016/j.xpro.2020.100091
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