Cargando…

Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells

The potential of reprogrammed β cells derived from pancreatic exocrine cells to treat diabetes has been demonstrated in animal models. However, the precise mechanisms and regulators involved in this process are not clear. Here, we describe a method that allows mechanistic studies of this process in...

Descripción completa

Detalles Bibliográficos
Autores principales: Elhanani, Ofer, Walker, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580220/
https://www.ncbi.nlm.nih.gov/pubmed/33111125
http://dx.doi.org/10.1016/j.xpro.2020.100096
_version_ 1783598746820411392
author Elhanani, Ofer
Walker, Michael D.
author_facet Elhanani, Ofer
Walker, Michael D.
author_sort Elhanani, Ofer
collection PubMed
description The potential of reprogrammed β cells derived from pancreatic exocrine cells to treat diabetes has been demonstrated in animal models. However, the precise mechanisms and regulators involved in this process are not clear. Here, we describe a method that allows mechanistic studies of this process in primary exocrine cultures using adenoviral expression vectors. This rapid 5-day protocol, provides the researcher with a highly controlled experimental system in which the effects of different compounds or genetic manipulations can be studied. For complete details on the use and execution of this protocol, please refer to Elhanani et al. (2020).
format Online
Article
Text
id pubmed-7580220
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75802202020-10-26 Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells Elhanani, Ofer Walker, Michael D. STAR Protoc Protocol The potential of reprogrammed β cells derived from pancreatic exocrine cells to treat diabetes has been demonstrated in animal models. However, the precise mechanisms and regulators involved in this process are not clear. Here, we describe a method that allows mechanistic studies of this process in primary exocrine cultures using adenoviral expression vectors. This rapid 5-day protocol, provides the researcher with a highly controlled experimental system in which the effects of different compounds or genetic manipulations can be studied. For complete details on the use and execution of this protocol, please refer to Elhanani et al. (2020). Elsevier 2020-08-28 /pmc/articles/PMC7580220/ /pubmed/33111125 http://dx.doi.org/10.1016/j.xpro.2020.100096 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Elhanani, Ofer
Walker, Michael D.
Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title_full Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title_fullStr Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title_full_unstemmed Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title_short Protocol for Studying Reprogramming of Mouse Pancreatic Acinar Cells to β-like Cells
title_sort protocol for studying reprogramming of mouse pancreatic acinar cells to β-like cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580220/
https://www.ncbi.nlm.nih.gov/pubmed/33111125
http://dx.doi.org/10.1016/j.xpro.2020.100096
work_keys_str_mv AT elhananiofer protocolforstudyingreprogrammingofmousepancreaticacinarcellstoblikecells
AT walkermichaeld protocolforstudyingreprogrammingofmousepancreaticacinarcellstoblikecells