Cargando…
Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum
Schistosoma japonicum (S. japonicum) is one of the etiological agents of schistosomiasis, a widespread zoonotic parasitic disease. However, the mechanism of the balanced co-existence between the host immune system and S. japonicum as well as their complex interaction remains unclear. In this study,...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580221/ https://www.ncbi.nlm.nih.gov/pubmed/33162984 http://dx.doi.org/10.3389/fimmu.2020.569727 |
_version_ | 1783598747087798272 |
---|---|
author | Hu, Yue Chen, Jiansong Xu, Yiyue Zhou, Hongli Huang, Ping Ma, Yubin Gao, Minzhao Cheng, Shaoyun Zhou, Haiyun Lv, Zhiyue |
author_facet | Hu, Yue Chen, Jiansong Xu, Yiyue Zhou, Hongli Huang, Ping Ma, Yubin Gao, Minzhao Cheng, Shaoyun Zhou, Haiyun Lv, Zhiyue |
author_sort | Hu, Yue |
collection | PubMed |
description | Schistosoma japonicum (S. japonicum) is one of the etiological agents of schistosomiasis, a widespread zoonotic parasitic disease. However, the mechanism of the balanced co-existence between the host immune system and S. japonicum as well as their complex interaction remains unclear. In this study, 16S rRNA gene sequencing, combined with metagenomic sequencing approach as well as ultraperformance liquid chromatography–mass spectrometry metabolic profiling, was applied to demonstrate changes in the gut microbiome community structure during schistosomiasis progression, the functional interactions between the gut bacteria and S. japonicum infection in BALB/c mice, and the dynamic metabolite changes of the host. The results showed that both gut microbiome and the metabolites were significantly altered at different time points after the infection. Decrease in richness and diversity as well as differed composition of the gut microbiota was observed in the infected status when compared with the uninfected status. At the phylum level, the gut microbial communities in all samples were dominated by Firmicutes, Bacteroidetes, Proteobacteria, and Deferribacteres, while at the genus level, Lactobacillus, Lachnospiraceae NK4A136 group, Bacteroides, Staphylococcus, and Alloprevotella were the most abundant. After exposure, Roseburia, and Ruminococcaceae UCG-014 decreased, while Staphylococcus, Alistipes, and Parabacteroides increased, which could raise the risk of infections. Furthermore, LEfSe demonstrated several bacterial taxa that could discriminate between each time point of S. japonicum infection. Besides that, metagenomic analysis illuminated that the AMP-activated protein kinase (AMPK) signaling pathway and the chemokine signaling pathway were significantly perturbed after the infection. Phosphatidylcholine and colfosceril palmitate in serum as well as xanthurenic acid, naphthalenesulfonic acid, and pimelylcarnitine in urine might be metabolic biomarkers due to their promising diagnostic potential at the early stage of the infection. Alterations of glycerophospholipid and purine metabolism were also discovered in the infection. The present study might provide further understanding of the mechanisms during schistosome infection in aspects of gut microbiome and metabolites, and facilitate the discovery of new targets for early diagnosis and prognostic purposes. Further validations of potential biomarkers in human populations are necessary, and the exploration of interactions among S. japonicum, gut microbiome, and metabolites is to be deepened in the future. |
format | Online Article Text |
id | pubmed-7580221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75802212020-11-05 Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum Hu, Yue Chen, Jiansong Xu, Yiyue Zhou, Hongli Huang, Ping Ma, Yubin Gao, Minzhao Cheng, Shaoyun Zhou, Haiyun Lv, Zhiyue Front Immunol Immunology Schistosoma japonicum (S. japonicum) is one of the etiological agents of schistosomiasis, a widespread zoonotic parasitic disease. However, the mechanism of the balanced co-existence between the host immune system and S. japonicum as well as their complex interaction remains unclear. In this study, 16S rRNA gene sequencing, combined with metagenomic sequencing approach as well as ultraperformance liquid chromatography–mass spectrometry metabolic profiling, was applied to demonstrate changes in the gut microbiome community structure during schistosomiasis progression, the functional interactions between the gut bacteria and S. japonicum infection in BALB/c mice, and the dynamic metabolite changes of the host. The results showed that both gut microbiome and the metabolites were significantly altered at different time points after the infection. Decrease in richness and diversity as well as differed composition of the gut microbiota was observed in the infected status when compared with the uninfected status. At the phylum level, the gut microbial communities in all samples were dominated by Firmicutes, Bacteroidetes, Proteobacteria, and Deferribacteres, while at the genus level, Lactobacillus, Lachnospiraceae NK4A136 group, Bacteroides, Staphylococcus, and Alloprevotella were the most abundant. After exposure, Roseburia, and Ruminococcaceae UCG-014 decreased, while Staphylococcus, Alistipes, and Parabacteroides increased, which could raise the risk of infections. Furthermore, LEfSe demonstrated several bacterial taxa that could discriminate between each time point of S. japonicum infection. Besides that, metagenomic analysis illuminated that the AMP-activated protein kinase (AMPK) signaling pathway and the chemokine signaling pathway were significantly perturbed after the infection. Phosphatidylcholine and colfosceril palmitate in serum as well as xanthurenic acid, naphthalenesulfonic acid, and pimelylcarnitine in urine might be metabolic biomarkers due to their promising diagnostic potential at the early stage of the infection. Alterations of glycerophospholipid and purine metabolism were also discovered in the infection. The present study might provide further understanding of the mechanisms during schistosome infection in aspects of gut microbiome and metabolites, and facilitate the discovery of new targets for early diagnosis and prognostic purposes. Further validations of potential biomarkers in human populations are necessary, and the exploration of interactions among S. japonicum, gut microbiome, and metabolites is to be deepened in the future. Frontiers Media S.A. 2020-10-08 /pmc/articles/PMC7580221/ /pubmed/33162984 http://dx.doi.org/10.3389/fimmu.2020.569727 Text en Copyright © 2020 Hu, Chen, Xu, Zhou, Huang, Ma, Gao, Cheng, Zhou and Lv. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Hu, Yue Chen, Jiansong Xu, Yiyue Zhou, Hongli Huang, Ping Ma, Yubin Gao, Minzhao Cheng, Shaoyun Zhou, Haiyun Lv, Zhiyue Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title | Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title_full | Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title_fullStr | Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title_full_unstemmed | Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title_short | Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum |
title_sort | alterations of gut microbiome and metabolite profiling in mice infected by schistosoma japonicum |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580221/ https://www.ncbi.nlm.nih.gov/pubmed/33162984 http://dx.doi.org/10.3389/fimmu.2020.569727 |
work_keys_str_mv | AT huyue alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT chenjiansong alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT xuyiyue alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT zhouhongli alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT huangping alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT mayubin alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT gaominzhao alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT chengshaoyun alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT zhouhaiyun alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum AT lvzhiyue alterationsofgutmicrobiomeandmetaboliteprofilinginmiceinfectedbyschistosomajaponicum |