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A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures

Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps fo...

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Detalles Bibliográficos
Autores principales: Teo, Jessica L., Lim, Chwee Teck, Yap, Alpha S., Saw, Thuan Beng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580222/
https://www.ncbi.nlm.nih.gov/pubmed/33111126
http://dx.doi.org/10.1016/j.xpro.2020.100098
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author Teo, Jessica L.
Lim, Chwee Teck
Yap, Alpha S.
Saw, Thuan Beng
author_facet Teo, Jessica L.
Lim, Chwee Teck
Yap, Alpha S.
Saw, Thuan Beng
author_sort Teo, Jessica L.
collection PubMed
description Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (2017) and Teo et al. (2020).
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spelling pubmed-75802222020-10-26 A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures Teo, Jessica L. Lim, Chwee Teck Yap, Alpha S. Saw, Thuan Beng STAR Protoc Protocol Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (2017) and Teo et al. (2020). Elsevier 2020-08-28 /pmc/articles/PMC7580222/ /pubmed/33111126 http://dx.doi.org/10.1016/j.xpro.2020.100098 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Teo, Jessica L.
Lim, Chwee Teck
Yap, Alpha S.
Saw, Thuan Beng
A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title_full A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title_fullStr A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title_full_unstemmed A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title_short A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
title_sort biologist’s guide to traction force microscopy using polydimethylsiloxane substrate for two-dimensional cell cultures
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580222/
https://www.ncbi.nlm.nih.gov/pubmed/33111126
http://dx.doi.org/10.1016/j.xpro.2020.100098
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