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A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures
Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps fo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580222/ https://www.ncbi.nlm.nih.gov/pubmed/33111126 http://dx.doi.org/10.1016/j.xpro.2020.100098 |
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author | Teo, Jessica L. Lim, Chwee Teck Yap, Alpha S. Saw, Thuan Beng |
author_facet | Teo, Jessica L. Lim, Chwee Teck Yap, Alpha S. Saw, Thuan Beng |
author_sort | Teo, Jessica L. |
collection | PubMed |
description | Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (2017) and Teo et al. (2020). |
format | Online Article Text |
id | pubmed-7580222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75802222020-10-26 A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures Teo, Jessica L. Lim, Chwee Teck Yap, Alpha S. Saw, Thuan Beng STAR Protoc Protocol Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps for analysis so readers can obtain traction forces to study the mechanobiology of epithelial cells. We also provide guidelines on when to adopt another common class of TFM protocols based on polyacrylamide hydrogels. For complete details on the use and execution of this protocol, please refer to Saw et al. (2017) and Teo et al. (2020). Elsevier 2020-08-28 /pmc/articles/PMC7580222/ /pubmed/33111126 http://dx.doi.org/10.1016/j.xpro.2020.100098 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Teo, Jessica L. Lim, Chwee Teck Yap, Alpha S. Saw, Thuan Beng A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title | A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title_full | A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title_fullStr | A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title_full_unstemmed | A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title_short | A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures |
title_sort | biologist’s guide to traction force microscopy using polydimethylsiloxane substrate for two-dimensional cell cultures |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580222/ https://www.ncbi.nlm.nih.gov/pubmed/33111126 http://dx.doi.org/10.1016/j.xpro.2020.100098 |
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