Processing Human Thymic Tissue for Single Cell RNA-Seq

Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34(+) progenitor and more differentiated CD34(–) fractions from post-natal thymic tissue to study thymopoiesis. CD34(+) cells represent &l...

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Detalles Bibliográficos
Autores principales: Le, Justin, Ha, Vi Luan, Luong, Annie, Parekh, Chintan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580228/
https://www.ncbi.nlm.nih.gov/pubmed/33111122
http://dx.doi.org/10.1016/j.xpro.2020.100090
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author Le, Justin
Ha, Vi Luan
Luong, Annie
Parekh, Chintan
author_facet Le, Justin
Ha, Vi Luan
Luong, Annie
Parekh, Chintan
author_sort Le, Justin
collection PubMed
description Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34(+) progenitor and more differentiated CD34(–) fractions from post-natal thymic tissue to study thymopoiesis. CD34(+) cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34(+) cells. For complete details on the use and execution of this protocol, please refer to Le et al. (2020).
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spelling pubmed-75802282020-10-26 Processing Human Thymic Tissue for Single Cell RNA-Seq Le, Justin Ha, Vi Luan Luong, Annie Parekh, Chintan STAR Protoc Protocol Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34(+) progenitor and more differentiated CD34(–) fractions from post-natal thymic tissue to study thymopoiesis. CD34(+) cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34(+) cells. For complete details on the use and execution of this protocol, please refer to Le et al. (2020). Elsevier 2020-08-24 /pmc/articles/PMC7580228/ /pubmed/33111122 http://dx.doi.org/10.1016/j.xpro.2020.100090 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Le, Justin
Ha, Vi Luan
Luong, Annie
Parekh, Chintan
Processing Human Thymic Tissue for Single Cell RNA-Seq
title Processing Human Thymic Tissue for Single Cell RNA-Seq
title_full Processing Human Thymic Tissue for Single Cell RNA-Seq
title_fullStr Processing Human Thymic Tissue for Single Cell RNA-Seq
title_full_unstemmed Processing Human Thymic Tissue for Single Cell RNA-Seq
title_short Processing Human Thymic Tissue for Single Cell RNA-Seq
title_sort processing human thymic tissue for single cell rna-seq
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580228/
https://www.ncbi.nlm.nih.gov/pubmed/33111122
http://dx.doi.org/10.1016/j.xpro.2020.100090
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