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Processing Human Thymic Tissue for Single Cell RNA-Seq

Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34(+) progenitor and more differentiated CD34(–) fractions from post-natal thymic tissue to study thymopoiesis. CD34(+) cells represent &l...

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Detalles Bibliográficos
Autores principales:	Le, Justin, Ha, Vi Luan, Luong, Annie, Parekh, Chintan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580228/ https://www.ncbi.nlm.nih.gov/pubmed/33111122 http://dx.doi.org/10.1016/j.xpro.2020.100090

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