Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells

Mammalian hematopoietic stem cells (HSCs) maintain life-long hematopoiesis in the bone marrow. HSCs remain quiescent in vivo, unlike more differentiated progenitors, and enter the cell cycle rapidly after bone marrow injury or in vitro culture. We have recently demonstrated the ability to maintain H...

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Detalles Bibliográficos
Autores principales: Kobayashi, Hiroshi, Takubo, Keiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580236/
https://www.ncbi.nlm.nih.gov/pubmed/33111112
http://dx.doi.org/10.1016/j.xpro.2020.100078
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author Kobayashi, Hiroshi
Takubo, Keiyo
author_facet Kobayashi, Hiroshi
Takubo, Keiyo
author_sort Kobayashi, Hiroshi
collection PubMed
description Mammalian hematopoietic stem cells (HSCs) maintain life-long hematopoiesis in the bone marrow. HSCs remain quiescent in vivo, unlike more differentiated progenitors, and enter the cell cycle rapidly after bone marrow injury or in vitro culture. We have recently demonstrated the ability to maintain HSC quiescence in vitro by mimicking the bone marrow microenvironment. Here, we provide a detailed protocol for keeping functional HSCs in the quiescent state in vitro. For complete details on the use and execution of this protocol, please refer to Kobayashi et al. (2019).
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spelling pubmed-75802362020-10-26 Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells Kobayashi, Hiroshi Takubo, Keiyo STAR Protoc Protocol Mammalian hematopoietic stem cells (HSCs) maintain life-long hematopoiesis in the bone marrow. HSCs remain quiescent in vivo, unlike more differentiated progenitors, and enter the cell cycle rapidly after bone marrow injury or in vitro culture. We have recently demonstrated the ability to maintain HSC quiescence in vitro by mimicking the bone marrow microenvironment. Here, we provide a detailed protocol for keeping functional HSCs in the quiescent state in vitro. For complete details on the use and execution of this protocol, please refer to Kobayashi et al. (2019). Elsevier 2020-08-02 /pmc/articles/PMC7580236/ /pubmed/33111112 http://dx.doi.org/10.1016/j.xpro.2020.100078 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kobayashi, Hiroshi
Takubo, Keiyo
Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title_full Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title_fullStr Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title_full_unstemmed Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title_short Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells
title_sort protocol for the maintenance of quiescent murine hematopoietic stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580236/
https://www.ncbi.nlm.nih.gov/pubmed/33111112
http://dx.doi.org/10.1016/j.xpro.2020.100078
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