Preparation and Analysis of GLOE-Seq Libraries for Genome-Wide Mapping of DNA Replication Patterns, Single-Strand Breaks, and Lesions

GLOE-Seq is a next-generation sequencing method for the genome-wide mapping of 3′-OH termini, either resulting from single- or double-strand breaks or introduced by enzymatic conversion of lesions or modified nucleotides. This protocol provides instructions for isolation of genomic DNA from budding...

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Detalles Bibliográficos
Autores principales: Petrosino, Giuseppe, Zilio, Nicola, Sriramachandran, Annie M., Ulrich, Helle D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580242/
https://www.ncbi.nlm.nih.gov/pubmed/33111111
http://dx.doi.org/10.1016/j.xpro.2020.100076

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