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Protocol for Barcoding T Cells Combined with Timed Stimulations
Stimulation of naive T lymphocytes via the T cell receptor (TCR) induces distinct phosphorylation patterns that can be used to explore various signaling pathways within the cell. This protocol can be used to characterize different perturbations to the signaling pathways and the variations in time of...
Autores principales: | Kulhanek, Kayla R., Myers, Darienne R., Ksionda, Olga, Vercoulen, Yvonne, Romero-Moya, Damia, Roose, Jeroen P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580249/ https://www.ncbi.nlm.nih.gov/pubmed/33111105 http://dx.doi.org/10.1016/j.xpro.2020.100067 |
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