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Combined Approach to Engineer a Highly Active Mutant of Processive Chitinase Hydrolyzing Crystalline Chitin

[Image: see text] Serratia marcescens chitinase A (SmChiA) processively hydrolyzes recalcitrant biomass crystalline chitin under mild conditions. Here, we combined multiple sequence alignment, site-saturation mutagenesis, and automated protein purification and activity measurement with liquid-handli...

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Detalles Bibliográficos
Autores principales:	Visootsat, Akasit, Nakamura, Akihiko, Wang, Tak-Wai, Iino, Ryota
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2020
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581260/ https://www.ncbi.nlm.nih.gov/pubmed/33111007 http://dx.doi.org/10.1021/acsomega.0c03911

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