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Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing

In this work, the development and testing of a novel fiber-optic based label-free biosensor is presented, whose performance were verified through the detection of C-reactive protein (CRP) in serum. The device is based on a long period grating fabricated in a double cladding fiber with a W-shaped ref...

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Autores principales: Esposito, Flavio, Sansone, Lucia, Srivastava, Anubhav, Baldini, Francesco, Campopiano, Stefania, Chiavaioli, Francesco, Giordano, Michele, Giannetti, Ambra, Iadicicco, Agostino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581380/
https://www.ncbi.nlm.nih.gov/pubmed/33129073
http://dx.doi.org/10.1016/j.bios.2020.112747
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author Esposito, Flavio
Sansone, Lucia
Srivastava, Anubhav
Baldini, Francesco
Campopiano, Stefania
Chiavaioli, Francesco
Giordano, Michele
Giannetti, Ambra
Iadicicco, Agostino
author_facet Esposito, Flavio
Sansone, Lucia
Srivastava, Anubhav
Baldini, Francesco
Campopiano, Stefania
Chiavaioli, Francesco
Giordano, Michele
Giannetti, Ambra
Iadicicco, Agostino
author_sort Esposito, Flavio
collection PubMed
description In this work, the development and testing of a novel fiber-optic based label-free biosensor is presented, whose performance were verified through the detection of C-reactive protein (CRP) in serum. The device is based on a long period grating fabricated in a double cladding fiber with a W-shaped refractive index (RI) profile. As a result, the working point of the device was tuned to the mode transition region by chemical etching of the outer fiber cladding, obtaining a significant enhancement of the RI sensitivity and an excellent visibility of the grating resonances due to the mode transition in an all-silica structure. The fiber transducer was coated with a nanometric thin layer of graphene oxide in order to provide functional groups for the covalent immobilization of the biological recognition element. A very low limit of detection of about 0.15 ng/mL was obtained during the detection of CRP in serum, and a large working range (1 ng/mL – 100 μg/mL) of clinical relevance has been also achieved.
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spelling pubmed-75813802020-10-23 Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing Esposito, Flavio Sansone, Lucia Srivastava, Anubhav Baldini, Francesco Campopiano, Stefania Chiavaioli, Francesco Giordano, Michele Giannetti, Ambra Iadicicco, Agostino Biosens Bioelectron Article In this work, the development and testing of a novel fiber-optic based label-free biosensor is presented, whose performance were verified through the detection of C-reactive protein (CRP) in serum. The device is based on a long period grating fabricated in a double cladding fiber with a W-shaped refractive index (RI) profile. As a result, the working point of the device was tuned to the mode transition region by chemical etching of the outer fiber cladding, obtaining a significant enhancement of the RI sensitivity and an excellent visibility of the grating resonances due to the mode transition in an all-silica structure. The fiber transducer was coated with a nanometric thin layer of graphene oxide in order to provide functional groups for the covalent immobilization of the biological recognition element. A very low limit of detection of about 0.15 ng/mL was obtained during the detection of CRP in serum, and a large working range (1 ng/mL – 100 μg/mL) of clinical relevance has been also achieved. Elsevier B.V. 2021-01-15 2020-10-22 /pmc/articles/PMC7581380/ /pubmed/33129073 http://dx.doi.org/10.1016/j.bios.2020.112747 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Esposito, Flavio
Sansone, Lucia
Srivastava, Anubhav
Baldini, Francesco
Campopiano, Stefania
Chiavaioli, Francesco
Giordano, Michele
Giannetti, Ambra
Iadicicco, Agostino
Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title_full Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title_fullStr Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title_full_unstemmed Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title_short Long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
title_sort long period grating in double cladding fiber coated with graphene oxide as high-performance optical platform for biosensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581380/
https://www.ncbi.nlm.nih.gov/pubmed/33129073
http://dx.doi.org/10.1016/j.bios.2020.112747
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