Cargando…

The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis

Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic...

Descripción completa

Detalles Bibliográficos
Autores principales: Borrelli, Mimi R., Patel, Ronak A., Adem, Sandeep, Diaz Deleon, Nestor M., Shen, Abra H., Sokol, Jan, Yen, Sara, Chang, Erin Y., Nazerali, Rahim, Nguyen, Dung, Momeni, Arash, Wang, Kevin C., Longaker, Michael T., Wan, Derrick C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581454/
https://www.ncbi.nlm.nih.gov/pubmed/32563212
http://dx.doi.org/10.1002/sctm.19-0317
_version_ 1783598982426001408
author Borrelli, Mimi R.
Patel, Ronak A.
Adem, Sandeep
Diaz Deleon, Nestor M.
Shen, Abra H.
Sokol, Jan
Yen, Sara
Chang, Erin Y.
Nazerali, Rahim
Nguyen, Dung
Momeni, Arash
Wang, Kevin C.
Longaker, Michael T.
Wan, Derrick C.
author_facet Borrelli, Mimi R.
Patel, Ronak A.
Adem, Sandeep
Diaz Deleon, Nestor M.
Shen, Abra H.
Sokol, Jan
Yen, Sara
Chang, Erin Y.
Nazerali, Rahim
Nguyen, Dung
Momeni, Arash
Wang, Kevin C.
Longaker, Michael T.
Wan, Derrick C.
author_sort Borrelli, Mimi R.
collection PubMed
description Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74− and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF‐β3) and decreased levels of TGF‐β1. Dermal fibroblasts incubated with conditioned media from CD74+ ASCs produced less collagen upon stimulation, compared to fibroblasts incubated with media from CD74− or US ASCs. Upon transplantation, fat grafts enriched with CD74+ ASCs reduced the stiffness, dermal thickness, and collagen content of overlying skin, and decreased the relative proportions of more fibrotic dermal fibroblasts. Improvements in several extracellular matrix components were also appreciated on immunofluorescent staining. Together these findings indicate CD74+ ASCs have antifibrotic qualities and may play an important role in future strategies to address fibrotic remodeling following radiation‐induced fibrosis.
format Online
Article
Text
id pubmed-7581454
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-75814542020-10-27 The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis Borrelli, Mimi R. Patel, Ronak A. Adem, Sandeep Diaz Deleon, Nestor M. Shen, Abra H. Sokol, Jan Yen, Sara Chang, Erin Y. Nazerali, Rahim Nguyen, Dung Momeni, Arash Wang, Kevin C. Longaker, Michael T. Wan, Derrick C. Stem Cells Transl Med Tissue Engineering and Regenerative Medicine Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74− and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF‐β3) and decreased levels of TGF‐β1. Dermal fibroblasts incubated with conditioned media from CD74+ ASCs produced less collagen upon stimulation, compared to fibroblasts incubated with media from CD74− or US ASCs. Upon transplantation, fat grafts enriched with CD74+ ASCs reduced the stiffness, dermal thickness, and collagen content of overlying skin, and decreased the relative proportions of more fibrotic dermal fibroblasts. Improvements in several extracellular matrix components were also appreciated on immunofluorescent staining. Together these findings indicate CD74+ ASCs have antifibrotic qualities and may play an important role in future strategies to address fibrotic remodeling following radiation‐induced fibrosis. John Wiley & Sons, Inc. 2020-06-20 /pmc/articles/PMC7581454/ /pubmed/32563212 http://dx.doi.org/10.1002/sctm.19-0317 Text en © 2020 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Tissue Engineering and Regenerative Medicine
Borrelli, Mimi R.
Patel, Ronak A.
Adem, Sandeep
Diaz Deleon, Nestor M.
Shen, Abra H.
Sokol, Jan
Yen, Sara
Chang, Erin Y.
Nazerali, Rahim
Nguyen, Dung
Momeni, Arash
Wang, Kevin C.
Longaker, Michael T.
Wan, Derrick C.
The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title_full The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title_fullStr The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title_full_unstemmed The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title_short The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
title_sort antifibrotic adipose‐derived stromal cell: grafted fat enriched with cd74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
topic Tissue Engineering and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581454/
https://www.ncbi.nlm.nih.gov/pubmed/32563212
http://dx.doi.org/10.1002/sctm.19-0317
work_keys_str_mv AT borrellimimir theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT patelronaka theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT ademsandeep theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT diazdeleonnestorm theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT shenabrah theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT sokoljan theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT yensara theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT changeriny theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT nazeralirahim theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT nguyendung theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT momeniarash theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT wangkevinc theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT longakermichaelt theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT wanderrickc theantifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT borrellimimir antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT patelronaka antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT ademsandeep antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT diazdeleonnestorm antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT shenabrah antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT sokoljan antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT yensara antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT changeriny antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT nazeralirahim antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT nguyendung antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT momeniarash antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT wangkevinc antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT longakermichaelt antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis
AT wanderrickc antifibroticadiposederivedstromalcellgraftedfatenrichedwithcd74adiposederivedstromalcellsreduceschronicradiationinducedskinfibrosis