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The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis
Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581454/ https://www.ncbi.nlm.nih.gov/pubmed/32563212 http://dx.doi.org/10.1002/sctm.19-0317 |
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author | Borrelli, Mimi R. Patel, Ronak A. Adem, Sandeep Diaz Deleon, Nestor M. Shen, Abra H. Sokol, Jan Yen, Sara Chang, Erin Y. Nazerali, Rahim Nguyen, Dung Momeni, Arash Wang, Kevin C. Longaker, Michael T. Wan, Derrick C. |
author_facet | Borrelli, Mimi R. Patel, Ronak A. Adem, Sandeep Diaz Deleon, Nestor M. Shen, Abra H. Sokol, Jan Yen, Sara Chang, Erin Y. Nazerali, Rahim Nguyen, Dung Momeni, Arash Wang, Kevin C. Longaker, Michael T. Wan, Derrick C. |
author_sort | Borrelli, Mimi R. |
collection | PubMed |
description | Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74− and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF‐β3) and decreased levels of TGF‐β1. Dermal fibroblasts incubated with conditioned media from CD74+ ASCs produced less collagen upon stimulation, compared to fibroblasts incubated with media from CD74− or US ASCs. Upon transplantation, fat grafts enriched with CD74+ ASCs reduced the stiffness, dermal thickness, and collagen content of overlying skin, and decreased the relative proportions of more fibrotic dermal fibroblasts. Improvements in several extracellular matrix components were also appreciated on immunofluorescent staining. Together these findings indicate CD74+ ASCs have antifibrotic qualities and may play an important role in future strategies to address fibrotic remodeling following radiation‐induced fibrosis. |
format | Online Article Text |
id | pubmed-7581454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75814542020-10-27 The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis Borrelli, Mimi R. Patel, Ronak A. Adem, Sandeep Diaz Deleon, Nestor M. Shen, Abra H. Sokol, Jan Yen, Sara Chang, Erin Y. Nazerali, Rahim Nguyen, Dung Momeni, Arash Wang, Kevin C. Longaker, Michael T. Wan, Derrick C. Stem Cells Transl Med Tissue Engineering and Regenerative Medicine Fat grafting can reduce radiation‐induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose‐derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74− and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF‐β3) and decreased levels of TGF‐β1. Dermal fibroblasts incubated with conditioned media from CD74+ ASCs produced less collagen upon stimulation, compared to fibroblasts incubated with media from CD74− or US ASCs. Upon transplantation, fat grafts enriched with CD74+ ASCs reduced the stiffness, dermal thickness, and collagen content of overlying skin, and decreased the relative proportions of more fibrotic dermal fibroblasts. Improvements in several extracellular matrix components were also appreciated on immunofluorescent staining. Together these findings indicate CD74+ ASCs have antifibrotic qualities and may play an important role in future strategies to address fibrotic remodeling following radiation‐induced fibrosis. John Wiley & Sons, Inc. 2020-06-20 /pmc/articles/PMC7581454/ /pubmed/32563212 http://dx.doi.org/10.1002/sctm.19-0317 Text en © 2020 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Tissue Engineering and Regenerative Medicine Borrelli, Mimi R. Patel, Ronak A. Adem, Sandeep Diaz Deleon, Nestor M. Shen, Abra H. Sokol, Jan Yen, Sara Chang, Erin Y. Nazerali, Rahim Nguyen, Dung Momeni, Arash Wang, Kevin C. Longaker, Michael T. Wan, Derrick C. The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title | The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title_full | The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title_fullStr | The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title_full_unstemmed | The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title_short | The antifibrotic adipose‐derived stromal cell: Grafted fat enriched with CD74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
title_sort | antifibrotic adipose‐derived stromal cell: grafted fat enriched with cd74+ adipose‐derived stromal cells reduces chronic radiation‐induced skin fibrosis |
topic | Tissue Engineering and Regenerative Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581454/ https://www.ncbi.nlm.nih.gov/pubmed/32563212 http://dx.doi.org/10.1002/sctm.19-0317 |
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