Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS

In recent years, advanced HPLC-MS strategies based on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) analysis have been implemented for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down analysis for polyclonal Ig...

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Detalles Bibliográficos
Autores principales: Blöchl, Constantin, Regl, Christof, Huber, Christian G., Winter, Petra, Weiss, Richard, Wohlschlager, Therese
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581757/
https://www.ncbi.nlm.nih.gov/pubmed/33093535
http://dx.doi.org/10.1038/s41598-020-75045-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581757/
https://www.ncbi.nlm.nih.gov/pubmed/33093535
http://dx.doi.org/10.1038/s41598-020-75045-1

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