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Inhibition of Orbivirus Replication by Aurintricarboxylic Acid

Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the Orbivirus genus, which are transmitted between hosts primarily by biting midges of the genus Culicoides. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there i...

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Autores principales: Alonso, Celia, Utrilla-Trigo, Sergio, Calvo-Pinilla, Eva, Jiménez-Cabello, Luis, Ortego, Javier, Nogales, Aitor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582255/
https://www.ncbi.nlm.nih.gov/pubmed/33023235
http://dx.doi.org/10.3390/ijms21197294
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author Alonso, Celia
Utrilla-Trigo, Sergio
Calvo-Pinilla, Eva
Jiménez-Cabello, Luis
Ortego, Javier
Nogales, Aitor
author_facet Alonso, Celia
Utrilla-Trigo, Sergio
Calvo-Pinilla, Eva
Jiménez-Cabello, Luis
Ortego, Javier
Nogales, Aitor
author_sort Alonso, Celia
collection PubMed
description Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the Orbivirus genus, which are transmitted between hosts primarily by biting midges of the genus Culicoides. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there is a pressing need for efficacious drugs to treat and control the spread of these infections. The polyanionic aromatic compound aurintricarboxylic acid (ATA) has been shown to have a broad-spectrum antiviral activity. Here, we evaluated ATA as a potential antiviral compound against Orbivirus infections in both mammalian and insect cells. Notably, ATA was able to prevent the replication of BTV and AHSV in both cell types in a time- and concentration-dependent manner. In addition, we evaluated the effect of ATA in vivo using a mouse model of infection. ATA did not protect mice against a lethal challenge with BTV or AHSV, most probably due to the in vivo effect of ATA on immune system regulation. Overall, these results demonstrate that ATA has inhibitory activity against Orbivirus replication in vitro, but further in vivo analysis will be required before considering it as a potential therapy for future clinical evaluation.
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spelling pubmed-75822552020-10-28 Inhibition of Orbivirus Replication by Aurintricarboxylic Acid Alonso, Celia Utrilla-Trigo, Sergio Calvo-Pinilla, Eva Jiménez-Cabello, Luis Ortego, Javier Nogales, Aitor Int J Mol Sci Article Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the Orbivirus genus, which are transmitted between hosts primarily by biting midges of the genus Culicoides. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there is a pressing need for efficacious drugs to treat and control the spread of these infections. The polyanionic aromatic compound aurintricarboxylic acid (ATA) has been shown to have a broad-spectrum antiviral activity. Here, we evaluated ATA as a potential antiviral compound against Orbivirus infections in both mammalian and insect cells. Notably, ATA was able to prevent the replication of BTV and AHSV in both cell types in a time- and concentration-dependent manner. In addition, we evaluated the effect of ATA in vivo using a mouse model of infection. ATA did not protect mice against a lethal challenge with BTV or AHSV, most probably due to the in vivo effect of ATA on immune system regulation. Overall, these results demonstrate that ATA has inhibitory activity against Orbivirus replication in vitro, but further in vivo analysis will be required before considering it as a potential therapy for future clinical evaluation. MDPI 2020-10-02 /pmc/articles/PMC7582255/ /pubmed/33023235 http://dx.doi.org/10.3390/ijms21197294 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alonso, Celia
Utrilla-Trigo, Sergio
Calvo-Pinilla, Eva
Jiménez-Cabello, Luis
Ortego, Javier
Nogales, Aitor
Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title_full Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title_fullStr Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title_full_unstemmed Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title_short Inhibition of Orbivirus Replication by Aurintricarboxylic Acid
title_sort inhibition of orbivirus replication by aurintricarboxylic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582255/
https://www.ncbi.nlm.nih.gov/pubmed/33023235
http://dx.doi.org/10.3390/ijms21197294
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