Cargando…
Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells
Extremely low frequency electromagnetic fields (ELF-EMFs) have been known to modulate inflammatory responses by targeting signal transduction pathways and influencing cellular redox balance through the generation of oxidants and antioxidants. Here, we studied the molecular mechanism underlying the a...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582394/ https://www.ncbi.nlm.nih.gov/pubmed/33023074 http://dx.doi.org/10.3390/ijms21197284 |
_version_ | 1783599181439434752 |
---|---|
author | Antonia, Patruno Erica, Costantini Alessio, Ferrone Mirko, Pesce Francesca, Diomede Oriana, Trubiani Marcella, Reale |
author_facet | Antonia, Patruno Erica, Costantini Alessio, Ferrone Mirko, Pesce Francesca, Diomede Oriana, Trubiani Marcella, Reale |
author_sort | Antonia, Patruno |
collection | PubMed |
description | Extremely low frequency electromagnetic fields (ELF-EMFs) have been known to modulate inflammatory responses by targeting signal transduction pathways and influencing cellular redox balance through the generation of oxidants and antioxidants. Here, we studied the molecular mechanism underlying the anti-oxidative effect of ELF-EMF in THP-1 cells, particularly with respect to antioxidant enzymes, such as heme oxygenase-1 (HO-1), regulated transcriptionally through nuclear factor E2-related factor 2 (Nrf2) activation. Cells treated with lipopolysaccharides (LPS) were exposed to a 50 Hz, 1 mT extremely low frequency electromagnetic fields for 1 h, 6 h and, 24 h. Our results indicate that ELF-EMF induced HO-1 mRNA and protein expression in LPS-treated THP-1 cells, with peak expression at 6 h, accompanied with a concomitant migration to the nucleus of a truncated HO-1 protein form. The immunostaining analysis further verified a nuclear enrichment of HO-1. Moreover, ELF-EMF inhibited the protein expressions of the sirtuin1 (SIRT1) and nuclear factor kappa B (NF-kB) pathways, confirming their anti-inflammatory/antioxidative role. Pretreatment with LY294002 (Akt inhibitor) and PD980559 (ERK inhibitor) inhibited LPS-induced Nrf2 nuclear translocation and HO-1 protein expression in ELF-EMF-exposed cells. Taken together, our results suggest that short ELF-EMF exposure exerts a protective role in THP-1 cells treated with an inflammatory/oxidative insult such as LPS, via the regulation of Nrf-2/HO-1 and SIRT1 /NF-kB pathways associated with intracellular glutathione (GSH) accumulation. |
format | Online Article Text |
id | pubmed-7582394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75823942020-10-29 Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells Antonia, Patruno Erica, Costantini Alessio, Ferrone Mirko, Pesce Francesca, Diomede Oriana, Trubiani Marcella, Reale Int J Mol Sci Article Extremely low frequency electromagnetic fields (ELF-EMFs) have been known to modulate inflammatory responses by targeting signal transduction pathways and influencing cellular redox balance through the generation of oxidants and antioxidants. Here, we studied the molecular mechanism underlying the anti-oxidative effect of ELF-EMF in THP-1 cells, particularly with respect to antioxidant enzymes, such as heme oxygenase-1 (HO-1), regulated transcriptionally through nuclear factor E2-related factor 2 (Nrf2) activation. Cells treated with lipopolysaccharides (LPS) were exposed to a 50 Hz, 1 mT extremely low frequency electromagnetic fields for 1 h, 6 h and, 24 h. Our results indicate that ELF-EMF induced HO-1 mRNA and protein expression in LPS-treated THP-1 cells, with peak expression at 6 h, accompanied with a concomitant migration to the nucleus of a truncated HO-1 protein form. The immunostaining analysis further verified a nuclear enrichment of HO-1. Moreover, ELF-EMF inhibited the protein expressions of the sirtuin1 (SIRT1) and nuclear factor kappa B (NF-kB) pathways, confirming their anti-inflammatory/antioxidative role. Pretreatment with LY294002 (Akt inhibitor) and PD980559 (ERK inhibitor) inhibited LPS-induced Nrf2 nuclear translocation and HO-1 protein expression in ELF-EMF-exposed cells. Taken together, our results suggest that short ELF-EMF exposure exerts a protective role in THP-1 cells treated with an inflammatory/oxidative insult such as LPS, via the regulation of Nrf-2/HO-1 and SIRT1 /NF-kB pathways associated with intracellular glutathione (GSH) accumulation. MDPI 2020-10-02 /pmc/articles/PMC7582394/ /pubmed/33023074 http://dx.doi.org/10.3390/ijms21197284 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Antonia, Patruno Erica, Costantini Alessio, Ferrone Mirko, Pesce Francesca, Diomede Oriana, Trubiani Marcella, Reale Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title | Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title_full | Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title_fullStr | Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title_full_unstemmed | Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title_short | Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells |
title_sort | short elf-emf exposure targets sirt1/nrf2/ho-1 signaling in thp-1 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582394/ https://www.ncbi.nlm.nih.gov/pubmed/33023074 http://dx.doi.org/10.3390/ijms21197284 |
work_keys_str_mv | AT antoniapatruno shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT ericacostantini shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT alessioferrone shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT mirkopesce shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT francescadiomede shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT orianatrubiani shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells AT marcellareale shortelfemfexposuretargetssirt1nrf2ho1signalinginthp1cells |