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A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus

A new thiopeptide (micrococcin P3, 1) and a known one (micrococcin P1, 2) were isolated from the culture broth of a marine-derived strain of Bacillus stratosphericus. The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolu...

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Autores principales: Wang, Weihong, Park, Kyu-Hyung, Lee, Jusung, Oh, Eunseok, Park, Chanyoon, Kang, Eunmo, Lee, Juni, Kang, Heonjoong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582574/
https://www.ncbi.nlm.nih.gov/pubmed/32987657
http://dx.doi.org/10.3390/molecules25194383
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author Wang, Weihong
Park, Kyu-Hyung
Lee, Jusung
Oh, Eunseok
Park, Chanyoon
Kang, Eunmo
Lee, Juni
Kang, Heonjoong
author_facet Wang, Weihong
Park, Kyu-Hyung
Lee, Jusung
Oh, Eunseok
Park, Chanyoon
Kang, Eunmo
Lee, Juni
Kang, Heonjoong
author_sort Wang, Weihong
collection PubMed
description A new thiopeptide (micrococcin P3, 1) and a known one (micrococcin P1, 2) were isolated from the culture broth of a marine-derived strain of Bacillus stratosphericus. The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolution mass spectrometry (HRMS), and tandem mass spectrometry. Both compounds exhibited potent antibacterial activities against Gram-positive strains with minimum inhibitory concentration (MIC) values of 0.05−0.8 μg/mL and did not show cytotoxicity in the MTT assay up to a concentration of 10 μM. This study adds a new promising member, micrococcin P3, to the family of thiopeptide antibiotics, which shows potential for the development of new antibiotics targeting Gram-positive bacteria.
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spelling pubmed-75825742020-10-28 A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus Wang, Weihong Park, Kyu-Hyung Lee, Jusung Oh, Eunseok Park, Chanyoon Kang, Eunmo Lee, Juni Kang, Heonjoong Molecules Communication A new thiopeptide (micrococcin P3, 1) and a known one (micrococcin P1, 2) were isolated from the culture broth of a marine-derived strain of Bacillus stratosphericus. The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolution mass spectrometry (HRMS), and tandem mass spectrometry. Both compounds exhibited potent antibacterial activities against Gram-positive strains with minimum inhibitory concentration (MIC) values of 0.05−0.8 μg/mL and did not show cytotoxicity in the MTT assay up to a concentration of 10 μM. This study adds a new promising member, micrococcin P3, to the family of thiopeptide antibiotics, which shows potential for the development of new antibiotics targeting Gram-positive bacteria. MDPI 2020-09-24 /pmc/articles/PMC7582574/ /pubmed/32987657 http://dx.doi.org/10.3390/molecules25194383 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wang, Weihong
Park, Kyu-Hyung
Lee, Jusung
Oh, Eunseok
Park, Chanyoon
Kang, Eunmo
Lee, Juni
Kang, Heonjoong
A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title_full A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title_fullStr A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title_full_unstemmed A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title_short A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus
title_sort new thiopeptide antibiotic, micrococcin p3, from a marine-derived strain of the bacterium bacillus stratosphericus
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582574/
https://www.ncbi.nlm.nih.gov/pubmed/32987657
http://dx.doi.org/10.3390/molecules25194383
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