New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii

Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs ta...

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Autores principales: Blanda, Valeria, D’Agostino, Rosalia, Giudice, Elisabetta, Randazzo, Kety, La Russa, Francesco, Villari, Sara, Vullo, Stefano, Torina, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582818/
https://www.ncbi.nlm.nih.gov/pubmed/32992475
http://dx.doi.org/10.3390/molecules25194431
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author Blanda, Valeria
D’Agostino, Rosalia
Giudice, Elisabetta
Randazzo, Kety
La Russa, Francesco
Villari, Sara
Vullo, Stefano
Torina, Alessandra
author_facet Blanda, Valeria
D’Agostino, Rosalia
Giudice, Elisabetta
Randazzo, Kety
La Russa, Francesco
Villari, Sara
Vullo, Stefano
Torina, Alessandra
author_sort Blanda, Valeria
collection PubMed
description Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species.
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spelling pubmed-75828182020-10-28 New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii Blanda, Valeria D’Agostino, Rosalia Giudice, Elisabetta Randazzo, Kety La Russa, Francesco Villari, Sara Vullo, Stefano Torina, Alessandra Molecules Article Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species. MDPI 2020-09-27 /pmc/articles/PMC7582818/ /pubmed/32992475 http://dx.doi.org/10.3390/molecules25194431 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blanda, Valeria
D’Agostino, Rosalia
Giudice, Elisabetta
Randazzo, Kety
La Russa, Francesco
Villari, Sara
Vullo, Stefano
Torina, Alessandra
New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title_full New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title_fullStr New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title_full_unstemmed New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title_short New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii
title_sort new real-time pcrs to differentiate rickettsia spp. and rickettsia conorii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582818/
https://www.ncbi.nlm.nih.gov/pubmed/32992475
http://dx.doi.org/10.3390/molecules25194431
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