Cargando…

Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica

Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Peng-Fei, Wang, Xun, Yang, Yu-Ying, You, Chun-Xiang, Zhang, Zhen-Lu, Hao, Yu-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582839/
https://www.ncbi.nlm.nih.gov/pubmed/33027937
http://dx.doi.org/10.3390/ijms21197350
_version_ 1783599282931105792
author Zheng, Peng-Fei
Wang, Xun
Yang, Yu-Ying
You, Chun-Xiang
Zhang, Zhen-Lu
Hao, Yu-Jin
author_facet Zheng, Peng-Fei
Wang, Xun
Yang, Yu-Ying
You, Chun-Xiang
Zhang, Zhen-Lu
Hao, Yu-Jin
author_sort Zheng, Peng-Fei
collection PubMed
description Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a worldwide perennial woody crop of economic importance. Here, seven PIF genes were identified from the Malus × domestica reference genome. Chromosomal locations, gene structures, and phylogenetic relationships of these members were analyzed. Analysis of cis-acting elements in promoter regions of MdPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. Subsequently, subcellular localization and transcriptional activity analysis revealed that MdPIFs were typical nuclear transcription factors with transcriptional activation ability. Expression analysis demonstrated that MdPIF genes had different gene expression patterns for various abiotic factors. Moreover, overexpressed MdPIF4 reduced the sensitivity of apple calluses to abscisic acid (ABA). Our work lays foundations for further investigation of PIF functions in plant growth and development in apples.
format Online
Article
Text
id pubmed-7582839
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75828392020-10-28 Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica Zheng, Peng-Fei Wang, Xun Yang, Yu-Ying You, Chun-Xiang Zhang, Zhen-Lu Hao, Yu-Jin Int J Mol Sci Article Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a worldwide perennial woody crop of economic importance. Here, seven PIF genes were identified from the Malus × domestica reference genome. Chromosomal locations, gene structures, and phylogenetic relationships of these members were analyzed. Analysis of cis-acting elements in promoter regions of MdPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. Subsequently, subcellular localization and transcriptional activity analysis revealed that MdPIFs were typical nuclear transcription factors with transcriptional activation ability. Expression analysis demonstrated that MdPIF genes had different gene expression patterns for various abiotic factors. Moreover, overexpressed MdPIF4 reduced the sensitivity of apple calluses to abscisic acid (ABA). Our work lays foundations for further investigation of PIF functions in plant growth and development in apples. MDPI 2020-10-05 /pmc/articles/PMC7582839/ /pubmed/33027937 http://dx.doi.org/10.3390/ijms21197350 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Peng-Fei
Wang, Xun
Yang, Yu-Ying
You, Chun-Xiang
Zhang, Zhen-Lu
Hao, Yu-Jin
Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title_full Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title_fullStr Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title_full_unstemmed Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title_short Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica
title_sort identification of phytochrome-interacting factor family members and functional analysis of mdpif4 in malus domestica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582839/
https://www.ncbi.nlm.nih.gov/pubmed/33027937
http://dx.doi.org/10.3390/ijms21197350
work_keys_str_mv AT zhengpengfei identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica
AT wangxun identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica
AT yangyuying identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica
AT youchunxiang identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica
AT zhangzhenlu identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica
AT haoyujin identificationofphytochromeinteractingfactorfamilymembersandfunctionalanalysisofmdpif4inmalusdomestica