Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells

Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells. Recently, we generated induced tissue-specific stem cells from pancreas (iTS-P cells) using reprogramming factors and tissue-specific selection. The iTS-P cells have self-renewal potential...

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Autores principales: Miyagi-Shiohira, Chika, Saitoh, Issei, Watanabe, Masami, Noguchi, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582910/
https://www.ncbi.nlm.nih.gov/pubmed/33093580
http://dx.doi.org/10.1038/s41598-020-75016-6
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author Miyagi-Shiohira, Chika
Saitoh, Issei
Watanabe, Masami
Noguchi, Hirofumi
author_facet Miyagi-Shiohira, Chika
Saitoh, Issei
Watanabe, Masami
Noguchi, Hirofumi
author_sort Miyagi-Shiohira, Chika
collection PubMed
description Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells. Recently, we generated induced tissue-specific stem cells from pancreas (iTS-P cells) using reprogramming factors and tissue-specific selection. The iTS-P cells have self-renewal potential, and subcutaneously transplanting them into immunodeficient mice did not generate teratomas. In this study, we applied KP-1 to analyze mouse ES (mES) cells and mouse iTS-P (miTS-P) cells. KP-1 completely stained mES cells in colonies, but only miTS-P cells at the edge of a colony. This difference was caused by cell type-specific expression of different ABC transporters. These finding suggest that KP-1 will be useful for distinguishing between iPS and iTS-P cells.
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spelling pubmed-75829102020-10-23 Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells Miyagi-Shiohira, Chika Saitoh, Issei Watanabe, Masami Noguchi, Hirofumi Sci Rep Article Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells. Recently, we generated induced tissue-specific stem cells from pancreas (iTS-P cells) using reprogramming factors and tissue-specific selection. The iTS-P cells have self-renewal potential, and subcutaneously transplanting them into immunodeficient mice did not generate teratomas. In this study, we applied KP-1 to analyze mouse ES (mES) cells and mouse iTS-P (miTS-P) cells. KP-1 completely stained mES cells in colonies, but only miTS-P cells at the edge of a colony. This difference was caused by cell type-specific expression of different ABC transporters. These finding suggest that KP-1 will be useful for distinguishing between iPS and iTS-P cells. Nature Publishing Group UK 2020-10-22 /pmc/articles/PMC7582910/ /pubmed/33093580 http://dx.doi.org/10.1038/s41598-020-75016-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Miyagi-Shiohira, Chika
Saitoh, Issei
Watanabe, Masami
Noguchi, Hirofumi
Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title_full Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title_fullStr Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title_full_unstemmed Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title_short Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
title_sort kyoto probe-1 reveals phenotypic differences between mouse es cells and its-p cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582910/
https://www.ncbi.nlm.nih.gov/pubmed/33093580
http://dx.doi.org/10.1038/s41598-020-75016-6
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