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Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance

MicroRNAs (miRNAs) and natural antisense transcripts (NATs) control many biological processes and have been broadly applied for genetic manipulation of eukaryotic gene expression. Still unclear, however, are whether and how NATs regulate miRNA production. Here, we report that the cis-NATs of MIR398...

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Autores principales: Li, Yajie, Li, Xiaorong, Yang, Jun, He, Yuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582911/
https://www.ncbi.nlm.nih.gov/pubmed/33093449
http://dx.doi.org/10.1038/s41467-020-19186-x
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author Li, Yajie
Li, Xiaorong
Yang, Jun
He, Yuke
author_facet Li, Yajie
Li, Xiaorong
Yang, Jun
He, Yuke
author_sort Li, Yajie
collection PubMed
description MicroRNAs (miRNAs) and natural antisense transcripts (NATs) control many biological processes and have been broadly applied for genetic manipulation of eukaryotic gene expression. Still unclear, however, are whether and how NATs regulate miRNA production. Here, we report that the cis-NATs of MIR398 genes repress the processing of their pri-miRNAs. Through genome-wide analysis of RNA sequencing data, we identify cis-NATs of MIRNA genes in Arabidopsis and Brassica. In Arabidopsis, MIR398b and MIR398c are coexpressed in vascular tissues with their antisense genes NAT398b and NAT398c, respectively. Knock down of NAT398b and NAT398c promotes miR398 processing, resulting in stronger plant thermotolerance owing to silencing of miR398-targeted genes; in contrast, their overexpression activates NAT398b and NAT398c, causing poorer thermotolerance due to the upregulation of miR398-targeted genes. Unexpectedly, overexpression of MIR398b and MIR398c activates NAT398b and NAT398c. Taken together, these results suggest that NAT398b/c repress miR398 biogenesis and attenuate plant thermotolerance via a regulatory loop.
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spelling pubmed-75829112020-10-29 Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance Li, Yajie Li, Xiaorong Yang, Jun He, Yuke Nat Commun Article MicroRNAs (miRNAs) and natural antisense transcripts (NATs) control many biological processes and have been broadly applied for genetic manipulation of eukaryotic gene expression. Still unclear, however, are whether and how NATs regulate miRNA production. Here, we report that the cis-NATs of MIR398 genes repress the processing of their pri-miRNAs. Through genome-wide analysis of RNA sequencing data, we identify cis-NATs of MIRNA genes in Arabidopsis and Brassica. In Arabidopsis, MIR398b and MIR398c are coexpressed in vascular tissues with their antisense genes NAT398b and NAT398c, respectively. Knock down of NAT398b and NAT398c promotes miR398 processing, resulting in stronger plant thermotolerance owing to silencing of miR398-targeted genes; in contrast, their overexpression activates NAT398b and NAT398c, causing poorer thermotolerance due to the upregulation of miR398-targeted genes. Unexpectedly, overexpression of MIR398b and MIR398c activates NAT398b and NAT398c. Taken together, these results suggest that NAT398b/c repress miR398 biogenesis and attenuate plant thermotolerance via a regulatory loop. Nature Publishing Group UK 2020-10-22 /pmc/articles/PMC7582911/ /pubmed/33093449 http://dx.doi.org/10.1038/s41467-020-19186-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Yajie
Li, Xiaorong
Yang, Jun
He, Yuke
Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title_full Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title_fullStr Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title_full_unstemmed Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title_short Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
title_sort natural antisense transcripts of mir398 genes suppress micror398 processing and attenuate plant thermotolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582911/
https://www.ncbi.nlm.nih.gov/pubmed/33093449
http://dx.doi.org/10.1038/s41467-020-19186-x
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