Cargando…

High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi

This investigation included the chemical analysis of Peganum harmala (P. harmala) seed oil and its antifungal properties against 10 fungal species. Seed oils of six populations were analyzed using high performance liquid chromatography (HPLC) and gas chromatograph/mass spectrometry (GC-MS). The HPLC...

Descripción completa

Detalles Bibliográficos
Autores principales: Hajji, Abdelhamid, Bnejdi, Fethi, Saadoun, Mourad, Ben Salem, Ibtissem, Nehdi, Imededdine, Sbihi, Hassen, Alharthi, Fahad A., El Bok, Safia, Boughalleb-M’Hamdi, Naima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582963/
https://www.ncbi.nlm.nih.gov/pubmed/33036316
http://dx.doi.org/10.3390/molecules25194569
_version_ 1783599311817277440
author Hajji, Abdelhamid
Bnejdi, Fethi
Saadoun, Mourad
Ben Salem, Ibtissem
Nehdi, Imededdine
Sbihi, Hassen
Alharthi, Fahad A.
El Bok, Safia
Boughalleb-M’Hamdi, Naima
author_facet Hajji, Abdelhamid
Bnejdi, Fethi
Saadoun, Mourad
Ben Salem, Ibtissem
Nehdi, Imededdine
Sbihi, Hassen
Alharthi, Fahad A.
El Bok, Safia
Boughalleb-M’Hamdi, Naima
author_sort Hajji, Abdelhamid
collection PubMed
description This investigation included the chemical analysis of Peganum harmala (P. harmala) seed oil and its antifungal properties against 10 fungal species. Seed oils of six populations were analyzed using high performance liquid chromatography (HPLC) and gas chromatograph/mass spectrometry (GC-MS). The HPLC analysis indicated that P. harmala seed oil exhibited a very high level of tocopherol contents, with values in the range of 2385.66–2722.68 mg/100 g. The most abundant tocopherol isomer was δ-tocopherol (90.39%), followed by γ-tocopherol (8.08%) and α-tocopherol (1.14%). We discovered for the first time the presence of tocotrenols in P. harmala seed oils of the six populations studied. The GC-MS analyses revealed that linoleic acid was the main fatty acid (65.17%), followed by oleic acid (23.12%), palmitic acid (5.36%) and stearic acid (3.08%). We also studied the antifungal activity of seed oil of the Medenine (MD) population on ten fungal pathogens. The antifungal effects differed among pathogens and depended on oil concentrations. Seed oil of the MD population caused a significant decrease in mycelial growth of all fungi tested, with values ranging 31.50–82.11%, except for Alternaria sp., which showed no inhibition. The antifungal activity against the 10 selected fungi can be explained by the richness in tocols of the extracted oil and make P. harmala a promising crop for biological control. Furthermore, the importance of fatty acids and the wide geographic spread in Tunisia of this species make this crop a potential source of renewable energy.
format Online
Article
Text
id pubmed-7582963
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75829632020-10-28 High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi Hajji, Abdelhamid Bnejdi, Fethi Saadoun, Mourad Ben Salem, Ibtissem Nehdi, Imededdine Sbihi, Hassen Alharthi, Fahad A. El Bok, Safia Boughalleb-M’Hamdi, Naima Molecules Article This investigation included the chemical analysis of Peganum harmala (P. harmala) seed oil and its antifungal properties against 10 fungal species. Seed oils of six populations were analyzed using high performance liquid chromatography (HPLC) and gas chromatograph/mass spectrometry (GC-MS). The HPLC analysis indicated that P. harmala seed oil exhibited a very high level of tocopherol contents, with values in the range of 2385.66–2722.68 mg/100 g. The most abundant tocopherol isomer was δ-tocopherol (90.39%), followed by γ-tocopherol (8.08%) and α-tocopherol (1.14%). We discovered for the first time the presence of tocotrenols in P. harmala seed oils of the six populations studied. The GC-MS analyses revealed that linoleic acid was the main fatty acid (65.17%), followed by oleic acid (23.12%), palmitic acid (5.36%) and stearic acid (3.08%). We also studied the antifungal activity of seed oil of the Medenine (MD) population on ten fungal pathogens. The antifungal effects differed among pathogens and depended on oil concentrations. Seed oil of the MD population caused a significant decrease in mycelial growth of all fungi tested, with values ranging 31.50–82.11%, except for Alternaria sp., which showed no inhibition. The antifungal activity against the 10 selected fungi can be explained by the richness in tocols of the extracted oil and make P. harmala a promising crop for biological control. Furthermore, the importance of fatty acids and the wide geographic spread in Tunisia of this species make this crop a potential source of renewable energy. MDPI 2020-10-06 /pmc/articles/PMC7582963/ /pubmed/33036316 http://dx.doi.org/10.3390/molecules25194569 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hajji, Abdelhamid
Bnejdi, Fethi
Saadoun, Mourad
Ben Salem, Ibtissem
Nehdi, Imededdine
Sbihi, Hassen
Alharthi, Fahad A.
El Bok, Safia
Boughalleb-M’Hamdi, Naima
High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title_full High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title_fullStr High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title_full_unstemmed High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title_short High Reserve in δ-Tocopherol of Peganum harmala Seeds Oil and Antifungal Activity of Oil against Ten Plant Pathogenic Fungi
title_sort high reserve in δ-tocopherol of peganum harmala seeds oil and antifungal activity of oil against ten plant pathogenic fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582963/
https://www.ncbi.nlm.nih.gov/pubmed/33036316
http://dx.doi.org/10.3390/molecules25194569
work_keys_str_mv AT hajjiabdelhamid highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT bnejdifethi highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT saadounmourad highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT bensalemibtissem highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT nehdiimededdine highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT sbihihassen highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT alharthifahada highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT elboksafia highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi
AT boughallebmhamdinaima highreserveindtocopherolofpeganumharmalaseedsoilandantifungalactivityofoilagainsttenplantpathogenicfungi