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Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model
Physiological Glucocorticoids are important regulators of the immune system. Pharmacological GCs are in widespread use to treat inflammatory diseases. Adrenalectomy (ADX) has been shown to exacerbate renal injury through inflammation and oxidative stress that results in renal impairment due to deple...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582976/ https://www.ncbi.nlm.nih.gov/pubmed/33007969 http://dx.doi.org/10.3390/molecules25194492 |
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author | Islam, Azim Ullah Shamsul Hellman, Björn Nyberg, Fred Amir, Naheed Jayaraj, Richard L. Petroianu, Georg Adem, Abdu |
author_facet | Islam, Azim Ullah Shamsul Hellman, Björn Nyberg, Fred Amir, Naheed Jayaraj, Richard L. Petroianu, Georg Adem, Abdu |
author_sort | Islam, Azim Ullah Shamsul |
collection | PubMed |
description | Physiological Glucocorticoids are important regulators of the immune system. Pharmacological GCs are in widespread use to treat inflammatory diseases. Adrenalectomy (ADX) has been shown to exacerbate renal injury through inflammation and oxidative stress that results in renal impairment due to depletion of GCs. In this study, the effect of myrcene to attenuate renal inflammation and oxidative stress was evaluated in the adrenalectomized rat model. Rats were adrenalectomized bilaterally or the adrenals were not removed after surgery (sham). Myrcene (50 mg/kg body weight, orally) was administered post ADX. Myrcene treatment resulted in significant downregulation of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) compared to untreated ADX rats. In addition, myrcene resulted in significant downregulation of immunomodulatory factors (IFNγ and NF-κB) and anti-inflammatory markers (IL-4 and IL-10) in treated ADX compared to untreated ADX. Myrcene significantly increased the antioxidant molecules (CAT, GSH, and SOD) and decreased MDA levels in treated ADX compared to untreated. Moreover, myrcene treatment reduced the expression of COX-2, iNOS, KIM-1, and kidney functional molecules (UREA, LDH, total protein, and creatinine) in ADX treated compared to ADX untreated. These results suggest that myrcene could be further developed as a therapeutic drug for treatment of kidney inflammation and injury. |
format | Online Article Text |
id | pubmed-7582976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75829762020-10-28 Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model Islam, Azim Ullah Shamsul Hellman, Björn Nyberg, Fred Amir, Naheed Jayaraj, Richard L. Petroianu, Georg Adem, Abdu Molecules Article Physiological Glucocorticoids are important regulators of the immune system. Pharmacological GCs are in widespread use to treat inflammatory diseases. Adrenalectomy (ADX) has been shown to exacerbate renal injury through inflammation and oxidative stress that results in renal impairment due to depletion of GCs. In this study, the effect of myrcene to attenuate renal inflammation and oxidative stress was evaluated in the adrenalectomized rat model. Rats were adrenalectomized bilaterally or the adrenals were not removed after surgery (sham). Myrcene (50 mg/kg body weight, orally) was administered post ADX. Myrcene treatment resulted in significant downregulation of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) compared to untreated ADX rats. In addition, myrcene resulted in significant downregulation of immunomodulatory factors (IFNγ and NF-κB) and anti-inflammatory markers (IL-4 and IL-10) in treated ADX compared to untreated ADX. Myrcene significantly increased the antioxidant molecules (CAT, GSH, and SOD) and decreased MDA levels in treated ADX compared to untreated. Moreover, myrcene treatment reduced the expression of COX-2, iNOS, KIM-1, and kidney functional molecules (UREA, LDH, total protein, and creatinine) in ADX treated compared to ADX untreated. These results suggest that myrcene could be further developed as a therapeutic drug for treatment of kidney inflammation and injury. MDPI 2020-09-30 /pmc/articles/PMC7582976/ /pubmed/33007969 http://dx.doi.org/10.3390/molecules25194492 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Islam, Azim Ullah Shamsul Hellman, Björn Nyberg, Fred Amir, Naheed Jayaraj, Richard L. Petroianu, Georg Adem, Abdu Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title | Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title_full | Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title_fullStr | Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title_full_unstemmed | Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title_short | Myrcene Attenuates Renal Inflammation and Oxidative Stress in the Adrenalectomized Rat Model |
title_sort | myrcene attenuates renal inflammation and oxidative stress in the adrenalectomized rat model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582976/ https://www.ncbi.nlm.nih.gov/pubmed/33007969 http://dx.doi.org/10.3390/molecules25194492 |
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