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Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs
Background: In addition to their roles in different biological processes, microRNAs in the tumor microenvironment appear to be potential diagnostic and prognostic biomarkers for various malignant diseases, including acute myeloid leukemia (AML). To date, no screening of circulating miRNAs has been c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583041/ https://www.ncbi.nlm.nih.gov/pubmed/32992819 http://dx.doi.org/10.3390/ijms21197065 |
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author | Moussa Agha, Douâa Rouas, Redouane Najar, Mehdi Bouhtit, Fatima Naamane, Najib Fayyad-Kazan, Hussein Bron, Dominique Meuleman, Nathalie Lewalle, Philippe Merimi, Makram |
author_facet | Moussa Agha, Douâa Rouas, Redouane Najar, Mehdi Bouhtit, Fatima Naamane, Najib Fayyad-Kazan, Hussein Bron, Dominique Meuleman, Nathalie Lewalle, Philippe Merimi, Makram |
author_sort | Moussa Agha, Douâa |
collection | PubMed |
description | Background: In addition to their roles in different biological processes, microRNAs in the tumor microenvironment appear to be potential diagnostic and prognostic biomarkers for various malignant diseases, including acute myeloid leukemia (AML). To date, no screening of circulating miRNAs has been carried out in the bone marrow compartment of AML. Accordingly, we investigated the circulating miRNA profile in AML bone marrow at diagnosis (AMLD) and first complete remission post treatment (AMLPT) in comparison to healthy donors (HD). Methods: Circulating miRNAs were isolated from AML bone marrow aspirations, and a low-density TaqMan miRNA array was performed to identify deregulated miRNAs followed by quantitative RT-PCR to validate the results. Bioinformatic analysis was conducted to evaluate the diagnostic and prognostic accuracy of the highly and significantly identified deregulated miRNA(s) as potential candidate biomarker(s). Results: We found several deregulated miRNAs between the AMLD vs. HD vs. AMLPT groups, which were involved in tumor progression and immune suppression pathways. We also identified significant diagnostic and prognostic signatures with the ability to predict AML patient treatment response. Conclusions: This study provides a possible role of enriched circulating bone marrow miRNAs in the initiation and progression of AML and highlights new markers for prognosis and treatment monitoring. |
format | Online Article Text |
id | pubmed-7583041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75830412020-10-28 Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs Moussa Agha, Douâa Rouas, Redouane Najar, Mehdi Bouhtit, Fatima Naamane, Najib Fayyad-Kazan, Hussein Bron, Dominique Meuleman, Nathalie Lewalle, Philippe Merimi, Makram Int J Mol Sci Article Background: In addition to their roles in different biological processes, microRNAs in the tumor microenvironment appear to be potential diagnostic and prognostic biomarkers for various malignant diseases, including acute myeloid leukemia (AML). To date, no screening of circulating miRNAs has been carried out in the bone marrow compartment of AML. Accordingly, we investigated the circulating miRNA profile in AML bone marrow at diagnosis (AMLD) and first complete remission post treatment (AMLPT) in comparison to healthy donors (HD). Methods: Circulating miRNAs were isolated from AML bone marrow aspirations, and a low-density TaqMan miRNA array was performed to identify deregulated miRNAs followed by quantitative RT-PCR to validate the results. Bioinformatic analysis was conducted to evaluate the diagnostic and prognostic accuracy of the highly and significantly identified deregulated miRNA(s) as potential candidate biomarker(s). Results: We found several deregulated miRNAs between the AMLD vs. HD vs. AMLPT groups, which were involved in tumor progression and immune suppression pathways. We also identified significant diagnostic and prognostic signatures with the ability to predict AML patient treatment response. Conclusions: This study provides a possible role of enriched circulating bone marrow miRNAs in the initiation and progression of AML and highlights new markers for prognosis and treatment monitoring. MDPI 2020-09-25 /pmc/articles/PMC7583041/ /pubmed/32992819 http://dx.doi.org/10.3390/ijms21197065 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moussa Agha, Douâa Rouas, Redouane Najar, Mehdi Bouhtit, Fatima Naamane, Najib Fayyad-Kazan, Hussein Bron, Dominique Meuleman, Nathalie Lewalle, Philippe Merimi, Makram Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title | Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title_full | Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title_fullStr | Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title_full_unstemmed | Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title_short | Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs |
title_sort | identification of acute myeloid leukemia bone marrow circulating micrornas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583041/ https://www.ncbi.nlm.nih.gov/pubmed/32992819 http://dx.doi.org/10.3390/ijms21197065 |
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