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Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model

BACKGROUND: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model. METHODS: In...

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Autores principales: Hasegawa, Hiro, Takeshita, Nobuyoshi, Ito, Masaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583199/
https://www.ncbi.nlm.nih.gov/pubmed/33092548
http://dx.doi.org/10.1186/s12893-020-00913-6
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author Hasegawa, Hiro
Takeshita, Nobuyoshi
Ito, Masaaki
author_facet Hasegawa, Hiro
Takeshita, Nobuyoshi
Ito, Masaaki
author_sort Hasegawa, Hiro
collection PubMed
description BACKGROUND: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model. METHODS: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO(2)) images. This technology can derive the StO(2) images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and 1 min and 30 min after the ligation of the cranial rectal artery (CRA). RESULTS: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO(2) level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO(2) level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO(2) level between 1 min after and 30 min after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively). CONCLUSION: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.
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spelling pubmed-75831992020-10-26 Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model Hasegawa, Hiro Takeshita, Nobuyoshi Ito, Masaaki BMC Surg Technical Advance BACKGROUND: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model. METHODS: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO(2)) images. This technology can derive the StO(2) images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and 1 min and 30 min after the ligation of the cranial rectal artery (CRA). RESULTS: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO(2) level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO(2) level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO(2) level between 1 min after and 30 min after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively). CONCLUSION: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model. BioMed Central 2020-10-22 /pmc/articles/PMC7583199/ /pubmed/33092548 http://dx.doi.org/10.1186/s12893-020-00913-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Technical Advance
Hasegawa, Hiro
Takeshita, Nobuyoshi
Ito, Masaaki
Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title_full Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title_fullStr Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title_full_unstemmed Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title_short Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
title_sort novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model
topic Technical Advance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583199/
https://www.ncbi.nlm.nih.gov/pubmed/33092548
http://dx.doi.org/10.1186/s12893-020-00913-6
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