A Colorimetric Assay for the Detection of Glucose and H(2)O(2) Based on Cu-Ag/g-C(3)N(4)/ZIF Hybrids with Superior Peroxidase Mimetic Activity

In this work, we report the synthesis of Cu-Ag bimetallic nanopartiles and g-C(3)N(4) nanosheets decorated on zeolitic imidazolate framework-8 (ZIF-8) to form a Cu-Ag/g-C(3)N(4)/ZIF hybrid. The hybrid was synthesized and characterized by Transmission electron microscopy (TEM), Fourier transformed infrared (FTIR), the X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The Cu-Ag/g-C(3)N(4)/ZIF hybrid has intrinsic peroxidaselike catalytic activity towards the oxidation of TMB in the presence of H(2)O(2). The situ synthesis of Cu-Ag bimetallic nanopartiles on 2D support such as g-C(3)N(4) nanosheets would significantly enhance the peroxidaselike catalytic properties of individual Cu-Ag bimetallic nanopartiles and the g-C(3)N(4) nanosheets. After loading of Cu-Ag bimetallic nanopartiles and g-C(3)N(4) nanosheets on the ZIF-8, the hybrids exhibited superior peroxidaselike catalytic activity and good recyclability. Then, this method was applied for detecting glucose in human serum, owing the significant potential for detection of metabolites with H(2)O(2)-generation reactions.