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Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection

To identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (1), 2′-hydroxyl neophellamuretin (2), yokovanol (3), swertisin (4), spinosin (5), and 7-methyl-apigenin-6-C-β-glucopyranosyl 2″-O-β-d-xylopyranoside (6) from MeOH extractions of Ohwia caudata. We s...

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Autores principales: Kwon, Eun Bin, Yang, Hye Jin, Choi, Jang-Gi, Li, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583851/
https://www.ncbi.nlm.nih.gov/pubmed/32987774
http://dx.doi.org/10.3390/molecules25194387
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author Kwon, Eun Bin
Yang, Hye Jin
Choi, Jang-Gi
Li, Wei
author_facet Kwon, Eun Bin
Yang, Hye Jin
Choi, Jang-Gi
Li, Wei
author_sort Kwon, Eun Bin
collection PubMed
description To identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (1), 2′-hydroxyl neophellamuretin (2), yokovanol (3), swertisin (4), spinosin (5), and 7-methyl-apigenin-6-C-β-glucopyranosyl 2″-O-β-d-xylopyranoside (6) from MeOH extractions of Ohwia caudata. We screened these compounds for antiviral activity using green fluorescent protein (GFP)-expressing H1N1 (A/PR/8/34) influenza A-infected RAW 264.7 cells. Compounds 1 and 3 exhibited significant inhibitory effects against influenza A viral infection in co-treatment conditions. In addition, compounds 1 and 3 reduced viral protein levels, including M1, M2, HA, and neuraminidase (NA), and suppressed neuraminidase (NA) activity in RAW 264.7 cells. These findings demonstrated that 2′-hydroxyl yokovanol and yokovanol, isolated from O. caudate, inhibit influenza A virus by suppressing NA activity. The moderate inhibitory activities of these flavonoids against influenza A virus suggest that they may be developed as novel anti-influenza drugs in the future.
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spelling pubmed-75838512020-10-28 Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection Kwon, Eun Bin Yang, Hye Jin Choi, Jang-Gi Li, Wei Molecules Communication To identify new potential anti-influenza compounds, we isolated six flavonoids, 2′-hydroxyl yokovanol (1), 2′-hydroxyl neophellamuretin (2), yokovanol (3), swertisin (4), spinosin (5), and 7-methyl-apigenin-6-C-β-glucopyranosyl 2″-O-β-d-xylopyranoside (6) from MeOH extractions of Ohwia caudata. We screened these compounds for antiviral activity using green fluorescent protein (GFP)-expressing H1N1 (A/PR/8/34) influenza A-infected RAW 264.7 cells. Compounds 1 and 3 exhibited significant inhibitory effects against influenza A viral infection in co-treatment conditions. In addition, compounds 1 and 3 reduced viral protein levels, including M1, M2, HA, and neuraminidase (NA), and suppressed neuraminidase (NA) activity in RAW 264.7 cells. These findings demonstrated that 2′-hydroxyl yokovanol and yokovanol, isolated from O. caudate, inhibit influenza A virus by suppressing NA activity. The moderate inhibitory activities of these flavonoids against influenza A virus suggest that they may be developed as novel anti-influenza drugs in the future. MDPI 2020-09-24 /pmc/articles/PMC7583851/ /pubmed/32987774 http://dx.doi.org/10.3390/molecules25194387 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kwon, Eun Bin
Yang, Hye Jin
Choi, Jang-Gi
Li, Wei
Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title_full Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title_fullStr Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title_full_unstemmed Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title_short Protective Effect of Flavonoids from Ohwia caudata against Influenza a Virus Infection
title_sort protective effect of flavonoids from ohwia caudata against influenza a virus infection
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583851/
https://www.ncbi.nlm.nih.gov/pubmed/32987774
http://dx.doi.org/10.3390/molecules25194387
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