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Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions

Mesenchymal stem cells (MSCs) are safe, and they have good therapeutic efficacy through their paracrine action. However, long-term culture to produce sufficient MSCs for clinical use can result in side-effects, such as an inevitable senescence and the reduction of the therapeutic efficacy of the MSC...

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Autores principales: Park, Sang Eon, Kim, Hyeongseop, Kwon, Soojin, Choi, Suk-joo, Oh, Soo-young, Ryu, Gyu Ha, Jeon, Hong Bae, Chang, Jong Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583852/
https://www.ncbi.nlm.nih.gov/pubmed/32993025
http://dx.doi.org/10.3390/ijms21197092
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author Park, Sang Eon
Kim, Hyeongseop
Kwon, Soojin
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
author_facet Park, Sang Eon
Kim, Hyeongseop
Kwon, Soojin
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
author_sort Park, Sang Eon
collection PubMed
description Mesenchymal stem cells (MSCs) are safe, and they have good therapeutic efficacy through their paracrine action. However, long-term culture to produce sufficient MSCs for clinical use can result in side-effects, such as an inevitable senescence and the reduction of the therapeutic efficacy of the MSCs. In order to overcome this, the primary culture conditions of the MSCs can be modified to simulate the stem cells’ niche environment, resulting in accelerated proliferation, the achievement of the target production yield at earlier passages, and the improvement of the therapeutic efficacy. We exposed Wharton’s jelly-derived MSCs (WJ-MSCs) to pressure stimuli during the primary culture step. In order to evaluate the proliferation, stemness, and therapeutic efficacy of WJ-MSCs, image, genetic, and Western blot analyses were carried out. Compared with standard incubation culture conditions, the cell proliferation was significantly improved when the WJ-MSCs were exposed to pressure stimuli. However, the therapeutic efficacy (the promotion of cell proliferation and anti-apoptotic effects) and the stemness of the WJ-MSCs was maintained, regardless of the culture conditions. Exposure to pressure stimuli is a simple and efficient way to improve WJ-MSC proliferation without causing changes in stemness and therapeutic efficacy. In this way, clinical-grade WJ-MSCs can be produced rapidly and used for therapeutic applications.
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spelling pubmed-75838522020-10-28 Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions Park, Sang Eon Kim, Hyeongseop Kwon, Soojin Choi, Suk-joo Oh, Soo-young Ryu, Gyu Ha Jeon, Hong Bae Chang, Jong Wook Int J Mol Sci Article Mesenchymal stem cells (MSCs) are safe, and they have good therapeutic efficacy through their paracrine action. However, long-term culture to produce sufficient MSCs for clinical use can result in side-effects, such as an inevitable senescence and the reduction of the therapeutic efficacy of the MSCs. In order to overcome this, the primary culture conditions of the MSCs can be modified to simulate the stem cells’ niche environment, resulting in accelerated proliferation, the achievement of the target production yield at earlier passages, and the improvement of the therapeutic efficacy. We exposed Wharton’s jelly-derived MSCs (WJ-MSCs) to pressure stimuli during the primary culture step. In order to evaluate the proliferation, stemness, and therapeutic efficacy of WJ-MSCs, image, genetic, and Western blot analyses were carried out. Compared with standard incubation culture conditions, the cell proliferation was significantly improved when the WJ-MSCs were exposed to pressure stimuli. However, the therapeutic efficacy (the promotion of cell proliferation and anti-apoptotic effects) and the stemness of the WJ-MSCs was maintained, regardless of the culture conditions. Exposure to pressure stimuli is a simple and efficient way to improve WJ-MSC proliferation without causing changes in stemness and therapeutic efficacy. In this way, clinical-grade WJ-MSCs can be produced rapidly and used for therapeutic applications. MDPI 2020-09-25 /pmc/articles/PMC7583852/ /pubmed/32993025 http://dx.doi.org/10.3390/ijms21197092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Sang Eon
Kim, Hyeongseop
Kwon, Soojin
Choi, Suk-joo
Oh, Soo-young
Ryu, Gyu Ha
Jeon, Hong Bae
Chang, Jong Wook
Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title_full Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title_fullStr Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title_full_unstemmed Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title_short Pressure Stimuli Improve the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions
title_sort pressure stimuli improve the proliferation of wharton’s jelly-derived mesenchymal stem cells under hypoxic culture conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583852/
https://www.ncbi.nlm.nih.gov/pubmed/32993025
http://dx.doi.org/10.3390/ijms21197092
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