Cargando…

The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients

MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism of genetic and epigenetic regulation of Treg and Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal...

Descripción completa

Detalles Bibliográficos
Autores principales: Kmiołek, Tomasz, Rzeszotarska, Ewa, Wajda, Anna, Walczuk, Ewa, Kuca-Warnawin, Ewa, Romanowska-Próchnicka, Katarzyna, Stypinska, Barbara, Majewski, Dominik, Jagodzinski, Pawel Piotr, Pawlik, Andrzej, Paradowska-Gorycka, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583886/
https://www.ncbi.nlm.nih.gov/pubmed/32998457
http://dx.doi.org/10.3390/ijms21197169
_version_ 1783599481508331520
author Kmiołek, Tomasz
Rzeszotarska, Ewa
Wajda, Anna
Walczuk, Ewa
Kuca-Warnawin, Ewa
Romanowska-Próchnicka, Katarzyna
Stypinska, Barbara
Majewski, Dominik
Jagodzinski, Pawel Piotr
Pawlik, Andrzej
Paradowska-Gorycka, Agnieszka
author_facet Kmiołek, Tomasz
Rzeszotarska, Ewa
Wajda, Anna
Walczuk, Ewa
Kuca-Warnawin, Ewa
Romanowska-Próchnicka, Katarzyna
Stypinska, Barbara
Majewski, Dominik
Jagodzinski, Pawel Piotr
Pawlik, Andrzej
Paradowska-Gorycka, Agnieszka
author_sort Kmiołek, Tomasz
collection PubMed
description MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism of genetic and epigenetic regulation of Treg and Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand the potential impact of the selected microRNAs expression profiles on Treg/Th17 cells frequency, RA phenotype, the expression profile of selected microRNAs, and their correlation with the expression profiles of selected transcriptional factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used osteoarthritis (OA) and healthy controls (HCs) as controls. The study was conducted on 14 RA and 11 OA patients, and 15 HCs. Treg/Th17 frequency was established by flow cytometry. Gene expression analysis was estimated by qPCR. We noticed correlations in RA Th17 cells between miR-26 and SMAD3, STAT3, SOCS1; and miR-155 and STAT3—and in RA Treg cells between miR-26 and SOCS1; miR-31, -155 and SMAD3; and miR-155 and SMAD4. In RA Tregs, we found a negative correlation between miR-26, -126 and STAT5a. The expression level of miR-31 in Th17 cells from RA patients with DAS28 ≤ 5.1 is higher and that for miR-24 is greater in Tregs from patients with DAS28 > 5.1. MiR-146a in Tregs is higher in rheumatoid factor (RF) positive RA patients.
format Online
Article
Text
id pubmed-7583886
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75838862020-10-29 The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients Kmiołek, Tomasz Rzeszotarska, Ewa Wajda, Anna Walczuk, Ewa Kuca-Warnawin, Ewa Romanowska-Próchnicka, Katarzyna Stypinska, Barbara Majewski, Dominik Jagodzinski, Pawel Piotr Pawlik, Andrzej Paradowska-Gorycka, Agnieszka Int J Mol Sci Article MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism of genetic and epigenetic regulation of Treg and Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand the potential impact of the selected microRNAs expression profiles on Treg/Th17 cells frequency, RA phenotype, the expression profile of selected microRNAs, and their correlation with the expression profiles of selected transcriptional factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used osteoarthritis (OA) and healthy controls (HCs) as controls. The study was conducted on 14 RA and 11 OA patients, and 15 HCs. Treg/Th17 frequency was established by flow cytometry. Gene expression analysis was estimated by qPCR. We noticed correlations in RA Th17 cells between miR-26 and SMAD3, STAT3, SOCS1; and miR-155 and STAT3—and in RA Treg cells between miR-26 and SOCS1; miR-31, -155 and SMAD3; and miR-155 and SMAD4. In RA Tregs, we found a negative correlation between miR-26, -126 and STAT5a. The expression level of miR-31 in Th17 cells from RA patients with DAS28 ≤ 5.1 is higher and that for miR-24 is greater in Tregs from patients with DAS28 > 5.1. MiR-146a in Tregs is higher in rheumatoid factor (RF) positive RA patients. MDPI 2020-09-28 /pmc/articles/PMC7583886/ /pubmed/32998457 http://dx.doi.org/10.3390/ijms21197169 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kmiołek, Tomasz
Rzeszotarska, Ewa
Wajda, Anna
Walczuk, Ewa
Kuca-Warnawin, Ewa
Romanowska-Próchnicka, Katarzyna
Stypinska, Barbara
Majewski, Dominik
Jagodzinski, Pawel Piotr
Pawlik, Andrzej
Paradowska-Gorycka, Agnieszka
The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title_full The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title_fullStr The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title_full_unstemmed The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title_short The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients
title_sort interplay between transcriptional factors and micrornas as an important factor for th17/treg balance in ra patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583886/
https://www.ncbi.nlm.nih.gov/pubmed/32998457
http://dx.doi.org/10.3390/ijms21197169
work_keys_str_mv AT kmiołektomasz theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT rzeszotarskaewa theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT wajdaanna theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT walczukewa theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT kucawarnawinewa theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT romanowskaprochnickakatarzyna theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT stypinskabarbara theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT majewskidominik theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT jagodzinskipawelpiotr theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT pawlikandrzej theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT paradowskagoryckaagnieszka theinterplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT kmiołektomasz interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT rzeszotarskaewa interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT wajdaanna interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT walczukewa interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT kucawarnawinewa interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT romanowskaprochnickakatarzyna interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT stypinskabarbara interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT majewskidominik interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT jagodzinskipawelpiotr interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT pawlikandrzej interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients
AT paradowskagoryckaagnieszka interplaybetweentranscriptionalfactorsandmicrornasasanimportantfactorforth17tregbalanceinrapatients