Cargando…

Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach

Our recent studies have demonstrated multiple health-promoting benefits from black walnut kernels. These biological functions of black walnuts are likely associated with their bioactive constituents. Characterization of phenolic compounds found in black walnut could point out underexplored bioactive...

Descripción completa

Detalles Bibliográficos
Autores principales: Ho, Khanh-Van, Roy, Anuradha, Foote, Sarah, Vo, Phuc H., Lall, Namrita, Lin, Chung-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583942/
https://www.ncbi.nlm.nih.gov/pubmed/33023106
http://dx.doi.org/10.3390/molecules25194516
_version_ 1783599494714097664
author Ho, Khanh-Van
Roy, Anuradha
Foote, Sarah
Vo, Phuc H.
Lall, Namrita
Lin, Chung-Ho
author_facet Ho, Khanh-Van
Roy, Anuradha
Foote, Sarah
Vo, Phuc H.
Lall, Namrita
Lin, Chung-Ho
author_sort Ho, Khanh-Van
collection PubMed
description Our recent studies have demonstrated multiple health-promoting benefits from black walnut kernels. These biological functions of black walnuts are likely associated with their bioactive constituents. Characterization of phenolic compounds found in black walnut could point out underexplored bioactive activities of black walnut extracts and promote the development of novel applications of black walnut and its by-products. In the present study, we assessed bioactivity profiles of phenolic compounds identified in the kernels of black walnuts using a high-throughput screening (HTS) approach. Black walnut phenolic compounds were evaluated in terms of their total antioxidant capacity, antioxidant response element (ARE) induction, and anticancer activities. The anticancer activities were identified by evaluating the effects of the phenolic compounds on the growth of the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). Out of 16 phenolic compounds tested, several compounds (penta-O-galloyl-β-d-glucose, epicatechin gallate, quercetin, (–)-epicatechin, rutin, quercetin 3-β-d-glucoside, gallic acid, (+)-catechin, ferulic acid, syringic acid) exerted antioxidant activities that were significantly higher compared to Trolox, which was used as a control. Two phenolic compounds, penta-O-galloyl-β-d-glucose and quercetin 3-β-d-glucoside, exhibited antiproliferative activities against both the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). The antioxidant activity of black walnut is likely driven not only by penta-O-galloyl-β-d-glucose but also by a combination of multiple phenolic compounds. Our findings suggested that black walnut extracts possibly possess anticancer activities and supported that penta-O-galloyl-β-d-glucose could be a potential bioactive agent for the cosmetic and pharmaceutical industries.
format Online
Article
Text
id pubmed-7583942
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75839422020-10-29 Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach Ho, Khanh-Van Roy, Anuradha Foote, Sarah Vo, Phuc H. Lall, Namrita Lin, Chung-Ho Molecules Article Our recent studies have demonstrated multiple health-promoting benefits from black walnut kernels. These biological functions of black walnuts are likely associated with their bioactive constituents. Characterization of phenolic compounds found in black walnut could point out underexplored bioactive activities of black walnut extracts and promote the development of novel applications of black walnut and its by-products. In the present study, we assessed bioactivity profiles of phenolic compounds identified in the kernels of black walnuts using a high-throughput screening (HTS) approach. Black walnut phenolic compounds were evaluated in terms of their total antioxidant capacity, antioxidant response element (ARE) induction, and anticancer activities. The anticancer activities were identified by evaluating the effects of the phenolic compounds on the growth of the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). Out of 16 phenolic compounds tested, several compounds (penta-O-galloyl-β-d-glucose, epicatechin gallate, quercetin, (–)-epicatechin, rutin, quercetin 3-β-d-glucoside, gallic acid, (+)-catechin, ferulic acid, syringic acid) exerted antioxidant activities that were significantly higher compared to Trolox, which was used as a control. Two phenolic compounds, penta-O-galloyl-β-d-glucose and quercetin 3-β-d-glucoside, exhibited antiproliferative activities against both the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). The antioxidant activity of black walnut is likely driven not only by penta-O-galloyl-β-d-glucose but also by a combination of multiple phenolic compounds. Our findings suggested that black walnut extracts possibly possess anticancer activities and supported that penta-O-galloyl-β-d-glucose could be a potential bioactive agent for the cosmetic and pharmaceutical industries. MDPI 2020-10-02 /pmc/articles/PMC7583942/ /pubmed/33023106 http://dx.doi.org/10.3390/molecules25194516 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ho, Khanh-Van
Roy, Anuradha
Foote, Sarah
Vo, Phuc H.
Lall, Namrita
Lin, Chung-Ho
Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title_full Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title_fullStr Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title_full_unstemmed Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title_short Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts (Juglans nigra) Using a High-Throughput Screening Approach
title_sort profiling anticancer and antioxidant activities of phenolic compounds present in black walnuts (juglans nigra) using a high-throughput screening approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583942/
https://www.ncbi.nlm.nih.gov/pubmed/33023106
http://dx.doi.org/10.3390/molecules25194516
work_keys_str_mv AT hokhanhvan profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach
AT royanuradha profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach
AT footesarah profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach
AT vophuch profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach
AT lallnamrita profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach
AT linchungho profilinganticancerandantioxidantactivitiesofphenoliccompoundspresentinblackwalnutsjuglansnigrausingahighthroughputscreeningapproach