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Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor bioavailability in the human body, caused by its low aqueous solubility and fast degradation, presents a big hu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584047/ https://www.ncbi.nlm.nih.gov/pubmed/33019782 http://dx.doi.org/10.3390/ijms21197276 |
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author | Arab-Tehrany, Elmira Elkhoury, Kamil Francius, Gregory Jierry, Loic Mano, Joao F. Kahn, Cyril Linder, Michel |
author_facet | Arab-Tehrany, Elmira Elkhoury, Kamil Francius, Gregory Jierry, Loic Mano, Joao F. Kahn, Cyril Linder, Michel |
author_sort | Arab-Tehrany, Elmira |
collection | PubMed |
description | Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor bioavailability in the human body, caused by its low aqueous solubility and fast degradation, presents a big hurdle for its oral administration. Here, we used nano-vesicles made of phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were encapsulated in the produced phospholipid system to form drug-loaded liposomes. Curcumin’s concentration was evaluated using UV-visible measurements. The maximal amount of curcumin that could be added to liposomes was assessed. Nuclear magnetic resonance (NMR) analyses were used to determine curcumin’s interactions and localization within the phospholipid membrane of the liposomes. X-ray scattering (SAXS) and atomic force microscopy (AFM) experiments were performed to characterize the membrane structure and organization, as well as its mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with the addition of curcumin in various amounts before saturation, allowing the preparation of a defined nanocarrier with desired amounts of the drug. |
format | Online Article Text |
id | pubmed-7584047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75840472020-10-29 Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization Arab-Tehrany, Elmira Elkhoury, Kamil Francius, Gregory Jierry, Loic Mano, Joao F. Kahn, Cyril Linder, Michel Int J Mol Sci Article Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor bioavailability in the human body, caused by its low aqueous solubility and fast degradation, presents a big hurdle for its oral administration. Here, we used nano-vesicles made of phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were encapsulated in the produced phospholipid system to form drug-loaded liposomes. Curcumin’s concentration was evaluated using UV-visible measurements. The maximal amount of curcumin that could be added to liposomes was assessed. Nuclear magnetic resonance (NMR) analyses were used to determine curcumin’s interactions and localization within the phospholipid membrane of the liposomes. X-ray scattering (SAXS) and atomic force microscopy (AFM) experiments were performed to characterize the membrane structure and organization, as well as its mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with the addition of curcumin in various amounts before saturation, allowing the preparation of a defined nanocarrier with desired amounts of the drug. MDPI 2020-10-01 /pmc/articles/PMC7584047/ /pubmed/33019782 http://dx.doi.org/10.3390/ijms21197276 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arab-Tehrany, Elmira Elkhoury, Kamil Francius, Gregory Jierry, Loic Mano, Joao F. Kahn, Cyril Linder, Michel Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title | Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title_full | Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title_fullStr | Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title_full_unstemmed | Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title_short | Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization |
title_sort | curcumin loaded nanoliposomes localization by nanoscale characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584047/ https://www.ncbi.nlm.nih.gov/pubmed/33019782 http://dx.doi.org/10.3390/ijms21197276 |
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