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Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hu...

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Autores principales: Arab-Tehrany, Elmira, Elkhoury, Kamil, Francius, Gregory, Jierry, Loic, Mano, Joao F., Kahn, Cyril, Linder, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584047/
https://www.ncbi.nlm.nih.gov/pubmed/33019782
http://dx.doi.org/10.3390/ijms21197276
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author Arab-Tehrany, Elmira
Elkhoury, Kamil
Francius, Gregory
Jierry, Loic
Mano, Joao F.
Kahn, Cyril
Linder, Michel
author_facet Arab-Tehrany, Elmira
Elkhoury, Kamil
Francius, Gregory
Jierry, Loic
Mano, Joao F.
Kahn, Cyril
Linder, Michel
author_sort Arab-Tehrany, Elmira
collection PubMed
description Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hurdle for its oral administration. Here, we used nano-vesicles made of ‎‎phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were ‎‎encapsulated in the produced phospholipid system to form drug-loaded liposomes. ‎Curcumin’s ‎concentration was evaluated using UV-visible measurements. The maximal ‎amount of curcumin ‎that could be added to liposomes was assessed. Nuclear magnetic ‎resonance (NMR) analyses ‎were used to determine curcumin’s interactions and localization ‎within the phospholipid ‎membrane of the liposomes. X-ray scattering (SAXS) and atomic ‎force microscopy (AFM) ‎experiments were performed to characterize the membrane structure ‎and organization, as well as its ‎mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with ‎the addition of curcumin in various ‎amounts before saturation, allowing the preparation of a ‎defined nanocarrier with desired ‎amounts of the drug.
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spelling pubmed-75840472020-10-29 Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization Arab-Tehrany, Elmira Elkhoury, Kamil Francius, Gregory Jierry, Loic Mano, Joao F. Kahn, Cyril Linder, Michel Int J Mol Sci Article Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hurdle for its oral administration. Here, we used nano-vesicles made of ‎‎phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were ‎‎encapsulated in the produced phospholipid system to form drug-loaded liposomes. ‎Curcumin’s ‎concentration was evaluated using UV-visible measurements. The maximal ‎amount of curcumin ‎that could be added to liposomes was assessed. Nuclear magnetic ‎resonance (NMR) analyses ‎were used to determine curcumin’s interactions and localization ‎within the phospholipid ‎membrane of the liposomes. X-ray scattering (SAXS) and atomic ‎force microscopy (AFM) ‎experiments were performed to characterize the membrane structure ‎and organization, as well as its ‎mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with ‎the addition of curcumin in various ‎amounts before saturation, allowing the preparation of a ‎defined nanocarrier with desired ‎amounts of the drug. MDPI 2020-10-01 /pmc/articles/PMC7584047/ /pubmed/33019782 http://dx.doi.org/10.3390/ijms21197276 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arab-Tehrany, Elmira
Elkhoury, Kamil
Francius, Gregory
Jierry, Loic
Mano, Joao F.
Kahn, Cyril
Linder, Michel
Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title_full Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title_fullStr Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title_full_unstemmed Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title_short Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
title_sort curcumin loaded nanoliposomes localization by nanoscale characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584047/
https://www.ncbi.nlm.nih.gov/pubmed/33019782
http://dx.doi.org/10.3390/ijms21197276
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